FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITYN OF DIFFERENT ORGANIC CRUDE EXTRACTS OF THE STEM OF SOLANUM NIGRUM L. BY ELECTROCHEMICAL METHOD

Abstract

The present research aimed to quantify the total flavonoid content and antioxidant properties in the different crude extract of the stem of Solanum nigrum that grow in southern Algeria. The results showed that EtOAc extract showed the highest  flavonoid content (18.410±0.023 mg QE/g). The electrochemical behavior of the chloroform, ethyl acetate and n-butanol extracts was studied and indicated that all extracts were electroactive and possess antioxidant activity, chloroform extract showed the highest capacity (9.58±0.010 mg/g). it has been shown that these samples showed scavenging ability on superoxide anion produced by electrochemical reduction of oxygen. The chloroform extract was the most efficient extract presenting the lowest IC50 values (0.664 mg/mL). The binding constant (Kb) of reaction and the change in free energy (ΔG) were calculated.