Species delimitation within the colonial flagellates Uroglena, Uroglenopsis and Urostipulosphaera (Chrysophyceae)

Abstract

ABSTRACT Until recently, there was no agreement on species delimitation within the morphologically similar chrysophycean genera Uroglena, Uroglenopsis and Urostipulosphaera. In this study, we aimed at a modern taxonomic revision based on the combination of morphological characters (ultrastructure of cysts, cell and colony features) and a multigene phylogeny (SSU, ITS rDNA and rbcL sequences), with ecology taken into account. Of more than 650 explored localities, only approximately one in 10 hosted a viable and detectable population of these colonial chrysophytes at the time of sampling. We established and examined 189 short-term cultures along with single colony isolates, derived mostly from blooming or encysting populations. We obtained the cyst morphology for four species and two lineages of Uroglena, two species of Uroglenopsis, and four species of Urostipulosphaera. A total of 12 resolved lineages could be attributed to previously described species or new species (Uroglena imitata sp. nov., Urostipulosphaera granulata sp. nov.). Based on our molecular analyses and morphological observations, we assign all the previously described Uroglena-like taxa to newly recognized genera and propose a key to identification. Consequently, Uroglena now includes 16 species and two varieties, Uroglenopsis contains four species and Urostipulosphaera encompasses nine species. Within Uroglena and Urostipulosphaera, species are defined by the ultrastructure of their cysts. On the contrary, as Uroglenopsis has simple cysts, species are defined by cell and colony characteristics.