Review—Systematic Review on Electrochemical Biosensing of Breast Cancer miRNAs to Develop Alternative DCIS Diagnostic Tool

Abstract

Till date, no systematic review is conducted on electrochemical biosensing of multiplexed breast cancer miRNAs to identify their suitability as an alternative diagnostic tool for ductal carcinoma in situ (DCIS). Original articles published in English from PUBMED, Science Direct, Scopus, MEDLINE, Cochrane Library, National Centre for Biotechnology Information, and Google scholar during Jan 2012–Feb 2022 were searched using set inclusion criteria. The Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) 2020 guidelines was followed to report this review. Methodological quality of the included studies was evaluated using Risk of Bias (ROB) assessment criteria. Out of 1973 screened articles, 17 studies were eligible and included in this review wherein, 1 study (5.88%) involving generic neutravidin modified nanolabel probe using biotintylated molecular beacons immobilized metal nanoparticles prepared using one pot assay to detect miRNAs 21 and 141 had low ROB and 16 studies (94.12%) had medium ROB. Medium ROB of majority of the included studies reveals its limited evidence to conclude its suitability for diagnosing DCIS using miRNAs. High quality studies with inter assays and validation are extensively needed for the development of diagnostic tool for DCIS via miRNAs.