Antimitotic effects of Cysticercus tenuicollis protoscolexes extract at administration to mice and their negative consequences for organism

Abstract

The purpose of the research is studying of Cysticercus tenuicollis protoscolexes extract effects on cell division at different routes of administration to mice and evaluation of the associated negative effects.Materials and methods. C. tenuicollis were obtained from spontaneously infected sheep in Kabardino-Balkarian Republic. C. tenuicollis protoscolexes were washed, crushed and homogenized. Protein extraction was performed with phosphate buffered saline pH 7.2–7.4. C. tenuicollis extract was administered intraperitoneally and intravenously to mice males at the dose level of 80 μg protein/animal. The control group of mice was intravenously injected with 0.1 ml of saline. At hours 3; 6; 24 and 48 post extract administration mice were euthanized. Bone marrow samples were taken from experimental and control mice for preparation of microscopic preparations to assess mitotic activity in a given cell population. The mitotic index was determined, all stages of mitosis were recorded. At the above time points blood samples were taken from mice to determine the main hematological parameters post intravenous and intraperitoneal administration of C. tenuicollis extract. The main hematological parameters of mice were determined using hematological analyzer MicroCC-20 Plus (High Technology, Inc. (USA)); leukocyte formula – by the generally accepted method. Samples of liver, kidneys, spleen, mesenteric lymph nodes and testes were taken from experimental and control animals for macroscopic and microscopic studies.Results and discussion. C. tenuicollis protoscolices extract leads to inhibition of cell division in the population bone marrow and testes cells in mice when administered intravenously and intraperitoneally at the dose level of 80 μg/animal manifested in accumulation of metaphases and decrease of other stages. At both routes of administration a decrease in leukocyte counts was noted. The observed microscopic changes in testes, spleen and lymph nodes either reflect the consequences of extract antimitotic effect or the immune response to the administration of C. tenuicollis extract.