Role of MEK1 and DIAPH3 Expression in Colorectal Carcinoma

A. Foda, Mohamed A. Ahmed, H. Elkalla, Eman El-zahaf, H. Abdallah, H. Wagih, M. Sami
{"title":"Role of MEK1 and DIAPH3 Expression in Colorectal Carcinoma","authors":"A. Foda, Mohamed A. Ahmed, H. Elkalla, Eman El-zahaf, H. Abdallah, H. Wagih, M. Sami","doi":"10.21608/RESONCOL.2018.4042.1059","DOIUrl":null,"url":null,"abstract":"Background: Colorectal carcinoma (CRC) is one of the serious causes of morbidity and mortality worldwide. It is characterized by activating mutations in genes encoding Receptor Tyrosine Kinases (RAS, RAF, MEK1 or MEK2) which act as driving oncogenes. DIAPH3 deficiency has been reported to enhance cancer cell motility, invasion and metastasis and also correlates with aggressive behaviour of cancer. Aim: To study the overexpression of MEK1 and DIAPH3 in CRC patients and their prognostic significance. Methods: We examined the immunohistochemical expression of MEK1 and DIAPH3 using tissue microarray technique in 150 CRC specimens divided into two groups. The mucinous group (MG) included specimens of 56 mucinous adenocarcinoma and 19 signet ring cell carcinoma, while the non-mucinous group (NMG) included 75 non-mucinous adenocarcinoma specimens for comparison. Results: MEK1 and DIAPH3 were strongly expressed in >50% of the studied specimens. The positivity of MEK1 expression was significantly higher in NMG compared to MG (66.7% and 34.3%, respectively; p<0.001). In all cases, the overexpression of MEK1 was significantly associated with peri-tumoral and intra-tumoral lymphocytic response (p=0.005 and 0.008, respectively). Furthermore, MEK1 overexpression showed statistically significant correlation with better OS (p=0.023) in the whole group of patients. The expression of DIAPH-3 did not differ significantly between NMG and MG (53.3% and 47.1%, respectively; p=0.456). There was strong relation between the overexpression of MEK1 and DIAPH3 (p<0.001). Conclusion: The results suggest a potential synergistic role of MEK1 and DIAPH3 overexpression and the development of CRC. Further large scale studies are warranted.","PeriodicalId":33915,"journal":{"name":"Research in Oncology","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in Oncology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/RESONCOL.2018.4042.1059","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3

Abstract

Background: Colorectal carcinoma (CRC) is one of the serious causes of morbidity and mortality worldwide. It is characterized by activating mutations in genes encoding Receptor Tyrosine Kinases (RAS, RAF, MEK1 or MEK2) which act as driving oncogenes. DIAPH3 deficiency has been reported to enhance cancer cell motility, invasion and metastasis and also correlates with aggressive behaviour of cancer. Aim: To study the overexpression of MEK1 and DIAPH3 in CRC patients and their prognostic significance. Methods: We examined the immunohistochemical expression of MEK1 and DIAPH3 using tissue microarray technique in 150 CRC specimens divided into two groups. The mucinous group (MG) included specimens of 56 mucinous adenocarcinoma and 19 signet ring cell carcinoma, while the non-mucinous group (NMG) included 75 non-mucinous adenocarcinoma specimens for comparison. Results: MEK1 and DIAPH3 were strongly expressed in >50% of the studied specimens. The positivity of MEK1 expression was significantly higher in NMG compared to MG (66.7% and 34.3%, respectively; p<0.001). In all cases, the overexpression of MEK1 was significantly associated with peri-tumoral and intra-tumoral lymphocytic response (p=0.005 and 0.008, respectively). Furthermore, MEK1 overexpression showed statistically significant correlation with better OS (p=0.023) in the whole group of patients. The expression of DIAPH-3 did not differ significantly between NMG and MG (53.3% and 47.1%, respectively; p=0.456). There was strong relation between the overexpression of MEK1 and DIAPH3 (p<0.001). Conclusion: The results suggest a potential synergistic role of MEK1 and DIAPH3 overexpression and the development of CRC. Further large scale studies are warranted.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
MEK1和DIAPH3在大肠癌中的表达
背景:结直肠癌(CRC)是全球发病率和死亡率的重要原因之一。其特征是激活编码受体酪氨酸激酶(RAS、RAF、MEK1或MEK2)的基因突变,这些基因起驱动致癌基因的作用。据报道,DIAPH3缺乏可增强癌症细胞的运动、侵袭和转移,并与癌症的侵袭行为相关。目的:研究MEK1和DIAPH3在CRC患者中的过度表达及其预后意义。方法:应用组织微阵列技术检测150例CRC组织中MEK1和DIAPH3的免疫组织化学表达。粘液组(MG)包括56例粘液腺癌和19例印戒细胞癌标本,而非粘液组(NMG)包括75例非粘液腺癌标本进行比较。结果:MEK1和DIAPH3在50%以上的研究标本中强烈表达。与MG相比,NMG中MEK1的表达阳性率显著更高(分别为66.7%和34.3%;p<0.001)。在所有病例中,MEK1的过度表达与肿瘤周围和肿瘤内淋巴细胞反应显著相关(分别为0.005和0.008)。此外,在整个患者组中,MEK1过表达与更好的OS显示出统计学上显著的相关性(p=0.023)。DIAPH-3的表达在NMG和MG之间没有显著差异(分别为53.3%和47.1%;p=0.456)。MEK1和DIAPH3的过表达之间存在强烈的相关性(p<0.001)。需要进行进一步的大规模研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
12
审稿时长
8 weeks
期刊最新文献
The Impact of Trastuzumab Administration Patterns on the Long-Term Clinical Outcomes of Patients with Non-Metastatic Breast Cancer in a Resource-Limited Setting Chordoid Meningioma with Extensive Lymphocytic Infiltration Forming Lymphoid Follicles: A Case Report Clinical Trials on Colorectal Cancer in South Africa The Influence of Serum Leptin Level and Body Mass Index on the Prognosis of Patients with Diffuse Large B Cell Lymphoma Multicentric vs. Unresectable Unicentric Castleman Disease with Active Presentation: An Orphan Rare Disease in a Young Egyptian Female Patient. A Case Report
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1