Two additional Colletotrichum species causing leaf spot of rambutan (Nephelium lappaceum)

IF 1 Q3 PLANT SCIENCES Archives of Phytopathology and Plant Protection Pub Date : 2023-03-16 DOI:10.1080/03235408.2023.2188993

E. Evallo, J. W. Taguiam, I. B. Posada, M. Balendres

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Abstract

Abstract Rambutan (Nephelium lappaceum) is an important fruit tree. Recently, leaf spots were observed in a tree in Los Baños, Laguna, Philippines. This study aims to identify the causal agent of rambutan leaf spot in the Philippines. Two fungal isolates, MBRAM001B and MBRAM003B, were isolated from the diseased leaves on potato dextrose agar medium. Colony growth and conidia characteristics of both isolates showed high resemblance with Colletotrichum species in the C. gloeosporioides species complex. Molecular characterisation of the isolates using the partial internal transcribed spacer rDNA and TUB2 regions identified the pathogens as C. tropicale MBRAM001B and C. siamense MBRAM003B. Leaf spots developed on rambutan leaves three days after inoculation and the same pathogens were isolated from the diseased leaves, establishing Koch’s Postulate. In conclusion, this study identified C. tropicale MBRAM001B and C. siamense MBRAM003B as causal agents of leaf spots in rambutan species in the Philippines. The survival and possible infection of these two Colletotrichum species on rambutan fruits and other fruit trees are worth further investigating.

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红毛丹(Nephelium lappaceum)叶斑病的两种炭疽菌

红毛丹(Nephelium lappaceum)是重要的果树。最近，在菲律宾拉古纳的洛斯Baños，一棵树上发现了叶子斑点。本研究旨在找出菲律宾红毛丹叶斑病的致病因子。在马铃薯葡萄糖琼脂培养基上，从马铃薯病叶中分离到2株真菌MBRAM001B和MBRAM003B。两个菌株的菌落生长和分生孢子特征与gloeosporioides种复合体中的炭疽菌种高度相似。利用部分内部转录间隔区rDNA和TUB2区对分离物进行分子鉴定，鉴定病原体为热带C. MBRAM001B和暹罗C. MBRAM003B。接种3天后红毛丹的叶片上出现了叶斑，并从患病叶片中分离出了相同的病原体，建立了科赫假设。综上所述，本研究确定了C. tropicale MBRAM001B和C. siamense MBRAM003B是菲律宾红毛丹种叶斑病的致病因子。这两种炭疽菌在红毛丹果实和其他果树上的存活和可能的侵染情况值得进一步研究。

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来源期刊

Archives of Phytopathology and Plant Protection Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.20

自引率

0.00%

发文量

100

期刊介绍： Archives of Phytopathology and Plant Protection publishes original papers and reviews covering all scientific aspects of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology, herbal studies and applied nematology and entomology as well as strategies and tactics of protecting crop plants and stocks of crop products against diseases. The journal provides a permanent forum for discussion of questions relating to the influence of plant protection measures on soil, water and air quality and on the fauna and flora, as well as to their interdependence in ecosystems of cultivated and neighbouring areas.