Comparative analysis of endophyte diversity of Dendrobium officinale lived on rock and tree.

Abstract

Dendrobium officinale usually lives on rock or tree, but their endophyte diversity has not yet been fully revealed? In this study, high-throughput sequencing technology was used to investigate the endophyte diversity of the roots of D. officinale lived on tree (Group 1-3, arboreal type) and rock (Group 4, lithophytic type). The results showed that their composition of endophytic fungi and bacteria were similar at phylum level, while their relative abundance were different. Their taxa composition and abundance of endophytes differed significantly among groups at the genus level. Alpha diversity of endophytic fungi of lithophytic type was higher than those from arboreal type, while there was no advantage in endophytic bacteria. Beta diversity revealed that the endophytic fungi tended to cluster in each group, but the endophytic bacteria were dispersed among the groups. LEfSe analysis found that the numbers of predicted endophyte biomarkers of lithophytic type were more than arboreal types at genus level, and the biomarkers varied among groups. Microbial network analysis revealed similarities and differences in the taxa composition and abundance of shared and special endophytes in each group. These results suggested that the root endophytes of lithophytic and arboreal D. officinale differed in diversity.