Fitorreguladores na calogênese e rizogênese em Eugenia involucrata

Abstract

This study evaluated the effect of growth regulators on callogenesis and rhizogenesis in Eugenia involucrata. In two experiments, we tested the naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP), and 2,4-dichlorophenoxyacetic acid (2,4-D) and BAP associations. After 45 days of culture, all treatments formed calli, however, those treatments with NAA (alone or combined with BAP) and 2,4-D combined with BAP (5:5 and 10:10 μM) presented the best results. Only the use of NAA at 10 μM induced rhizogenesis. At 90 days of culture, the effect of phytoregulators was most evident, where 2,4-D combined with BAP (5:5 and 10:10 μM) were more favorable for callogenesis while 10 μM NAA were more favorable for rhizogenesis. These results indicate that cell dedifferentiation and re-differentiation processes are achievable from leaf explants of Eugenia involucrata.