{"title":"Genome editing in mice and its application to the study of spermatogenesis","authors":"Seiya Oura , Hideto Mori , Masahito Ikawa","doi":"10.1016/j.ggedit.2022.100014","DOIUrl":null,"url":null,"abstract":"<div><p>Gene modification technology has long been beneficial for unraveling the mystery of biological phenomena. Thus, the advent of the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas) 9 system was a game-changer in biological research because of its efficiency and simplicity of design. This review article first describes a brief guideline of gRNA design and the methods to incorporate the CRISPR/Cas9 system into zygotes. Then, we will also discuss the application of this technology to the study of male reproductive biology, including knock-out (KO) phenotypical screening of genes expressed in the male reproductive tissues.</p></div>","PeriodicalId":73137,"journal":{"name":"Gene and genome editing","volume":"3 ","pages":"Article 100014"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666388022000041/pdfft?md5=c8b36d0f294a2356a30167622ac7c601&pid=1-s2.0-S2666388022000041-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene and genome editing","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666388022000041","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Gene modification technology has long been beneficial for unraveling the mystery of biological phenomena. Thus, the advent of the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR-associated (Cas) 9 system was a game-changer in biological research because of its efficiency and simplicity of design. This review article first describes a brief guideline of gRNA design and the methods to incorporate the CRISPR/Cas9 system into zygotes. Then, we will also discuss the application of this technology to the study of male reproductive biology, including knock-out (KO) phenotypical screening of genes expressed in the male reproductive tissues.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
