R. Latina, Clare Hazel R. Tabernilla, A. H. Ramirez
{"title":"Detection of root-knot nematodes infecting weedy rice in San Juan, Batangas, Philippines","authors":"R. Latina, Clare Hazel R. Tabernilla, A. H. Ramirez","doi":"10.1080/03235408.2023.2217675","DOIUrl":null,"url":null,"abstract":"Abstract Weedy rice collected from a rice field was found to be infected with root-knot nematodes. Perineal patterns of root-knot nematode females from galled weedy rice roots revealed Meloidogyne graminicola morphology with vulva-anus complex showing egg-like appearance, moderately high dorsal arch, smooth striae on the edge with distinct striations near the smooth vulval lips and absence of lateral ridges. DNA amplification based on M. graminicola-specific marker yielded positive detection. ITS-rDNA-based characterization revealed 99.81% similarity of the local isolate to M. graminicola sequences from NCBI GenBank®. ITS-based maximum-likelihood phylogram placed the isolate in M. graminicola clade with a high bootstrap support.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":"56 1","pages":"753 - 759"},"PeriodicalIF":1.0000,"publicationDate":"2023-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Phytopathology and Plant Protection","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/03235408.2023.2217675","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Abstract Weedy rice collected from a rice field was found to be infected with root-knot nematodes. Perineal patterns of root-knot nematode females from galled weedy rice roots revealed Meloidogyne graminicola morphology with vulva-anus complex showing egg-like appearance, moderately high dorsal arch, smooth striae on the edge with distinct striations near the smooth vulval lips and absence of lateral ridges. DNA amplification based on M. graminicola-specific marker yielded positive detection. ITS-rDNA-based characterization revealed 99.81% similarity of the local isolate to M. graminicola sequences from NCBI GenBank®. ITS-based maximum-likelihood phylogram placed the isolate in M. graminicola clade with a high bootstrap support.
期刊介绍：
Archives of Phytopathology and Plant Protection publishes original papers and reviews covering all scientific aspects of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology, herbal studies and applied nematology and entomology as well as strategies and tactics of protecting crop plants and stocks of crop products against diseases. The journal provides a permanent forum for discussion of questions relating to the influence of plant protection measures on soil, water and air quality and on the fauna and flora, as well as to their interdependence in ecosystems of cultivated and neighbouring areas.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
