K. Sobhi, Souhila Mélaïne, Abderrahmane Abdaoui, M. Mammar
{"title":"Toxicological screening method by HPLC-DAD with local spectral library and \"home-made\" internal standard","authors":"K. Sobhi, Souhila Mélaïne, Abderrahmane Abdaoui, M. Mammar","doi":"10.4314/jfas.1180","DOIUrl":null,"url":null,"abstract":"A liquid-liquid extraction technique and HPLC-DAD method using a C18 column, 5μm (150x4.6mm i.d.) were developed for a toxicological drug screening. A home-made “N-acetylparoxetine” is used as internal standard. A local UV spectra library of over 130 drugs and some of their metabolites was created. This library covers the main therapeutic classes involved in intoxication cases. The developed procedure is successfully applied to different biological samples (serum, urine, gastric fluid and post mortem whole blood) and allows rapid and simultaneous detection and identification of a large number of substances and their semi-quantification in serum.","PeriodicalId":15885,"journal":{"name":"Journal of Fundamental and Applied Sciences","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fundamental and Applied Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/jfas.1180","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A liquid-liquid extraction technique and HPLC-DAD method using a C18 column, 5μm (150x4.6mm i.d.) were developed for a toxicological drug screening. A home-made “N-acetylparoxetine” is used as internal standard. A local UV spectra library of over 130 drugs and some of their metabolites was created. This library covers the main therapeutic classes involved in intoxication cases. The developed procedure is successfully applied to different biological samples (serum, urine, gastric fluid and post mortem whole blood) and allows rapid and simultaneous detection and identification of a large number of substances and their semi-quantification in serum.
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