Production of hispidin polyphenols from medicinal mushroom Sanghuangporus vaninii in submerged cultures

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2023-10-01 DOI:10.1016/j.chmed.2022.07.004
Wenwen Yuan , Weihua Yuan , Rong Zhou , Guoying Lv , Mengni Sun , Yanxia Zhao , Weifa Zheng
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引用次数: 1

Abstract

Objective

The medicinal mushroom Sanghuangporus vaninii produces pharmaceutically valuable hispidin polyphenols in natural habitats. However, due to the slow growth in nature, S. vaninii grown in the field (sclerotia) is not reliable for pharmaceutical purposes. Although higher biomass of fungal mycelia can be obtained in submerged cultures, the accumulation of hispidin polyphenols is rare.

Methods

In this study, the polyunsaturated fatty acids (PUFAs), linoleic acid (LA), linolenic acid (ALA), and methyl jasmonate (MeJa) were employed as the stimulant agents to coordinate the accumulation of biomass and hispidin polyphenols in its submerged cultures.

Results

The addition of LA and ALA promoted the mycelial accumulation, while the addition of MeJa inhibited the growth of S. vaninii concomitant with reduced total polyphenols. UPLC-Triple-TOF-MS analysis revealed an increased production of hispidin, phellinstatin, pinnilidine, and its derivatives upon the addition of LA and ALA, and hypholomine B and its isomer, 3,14’-bihispidinyl, and phelligridin E upon the addition of MeJa on day 13. Intriguingly, total polyphenols from the MeJa-supplementing cultures harbored a high capacity in scavenging free radicals. Chemical structural analysis showed that hispidin polyphenols had higher antioxidant activity due to more hispidin moieties induced by MeJa.

Conclusion

The supplement of PUFAs affects the synthesis and composition of hispidin polyphenols in S. vaninii. Our results provide a possibility to coordinate the production of hispidin polyphenols via submerged cultures of S. vaninii.

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药用蘑菇桑黄菌深层发酵生产hispidin多酚
目的药用蘑菇桑黄茯苓在自然生境中产生具有药用价值的皂素多酚。然而,由于在自然界中生长缓慢,在野外生长的vaninii(菌核)不可靠的药用目的。虽然在深层培养中可以获得较高的真菌菌丝体生物量,但hispidin多酚的积累是罕见的。方法采用多不饱和脂肪酸(PUFAs)、亚油酸(LA)、亚麻酸(ALA)和茉莉酸甲酯(MeJa)作为刺激剂,协调其深层培养中生物量和hispidin多酚的积累。结果LA和ALA的添加促进了葡萄球菌菌丝的积累,而MeJa的添加抑制了葡萄球菌的生长,并降低了总多酚含量。uplc -三重tof - ms分析显示,在第13天添加LA和ALA后,毛毛苷、黑毛苷、pinnilidine及其衍生物的产量增加,添加MeJa后,毛毛胺B及其异构体3,14′-比利亚毛苷基和毛毛苷E的产量增加。有趣的是,从补充meja的培养物中获得的总多酚具有清除自由基的高能力。化学结构分析表明,MeJa诱导的hispidin多酚具有较高的抗氧化活性。结论补充PUFAs可影响鼠尾草中hispidin多酚的合成和组成。我们的结果提供了一种可能性,以协调生产hispidin多酚通过潜水培养s.vaninii。
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来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
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