Jade Pattyn , Severien Van Keer , Wiebren Tjalma , Veerle Matheeussen , Pierre Van Damme , Alex Vorsters
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引用次数: 30
Abstract
Background
Human papillomavirus (HPV) infects and propagates in the cervical mucosal epithelium. Hence, in addition to assessing systemic immunity, the accurate measurement of cervical immunity is important to evaluate local immune responses to HPV infection and vaccination. This review discusses studies that investigated the presence of infection and vaccine-induced HPV-specific antibodies in cervicovaginal secretions (CVS).
Methods
We searched the two main health sciences databases, PubMed and the ISI Web of Science, from the earliest dates available to March 2019. From the eligible publications, information was extracted regarding: (i) study design, (ii) the reported HPV-specific antibody concentrations in CVS (and the associated serum levels, when provided), (iii) the CVS collection method, and (iv) the immunoassays used.
Results
The systematic search and selection process yielded 44 articles. The evidence of HPV-specific antibodies in CVS after natural infection (26/44) and HPV vaccination (18/44) is discussed. Many studies indicate that HPV-specific antibody detection in CVS is variable but feasible with a variety of collection methods and immunoassays. Most CVS samples were collected by cervicovaginal washing or wicks, and antibody presence was mostly determined by VLP-based ELISAs. The moderate to strong correlation between vaccine-induced antibody levels in serum and in CVS indicates that HPV vaccines generate antibodies that transudate through the cervical mucosal epithelium.
Conclusion
Although HPV-specific antibodies have lower titres in CVS than in serum samples, studies have shown that their detection in CVS is feasible. Nevertheless, the high variability of published observations and the lack of a strictly uniform, well-validated method for the collection, isolation and quantification of antibodies indicates a need for specific methods to improve and standardize the detection of HPV-specific antibodies in CVS.
背景:人乳头瘤病毒(HPV)在宫颈粘膜上皮中感染和传播。因此,除了评估全身免疫外,宫颈免疫的准确测量对于评估局部对HPV感染和疫苗接种的免疫反应也很重要。本综述讨论了在宫颈阴道分泌物(CVS)中存在感染和疫苗诱导的hpv特异性抗体的研究。方法我们检索了两个主要的健康科学数据库,PubMed和ISI Web of Science,从最早的日期到2019年3月。从符合条件的出版物中提取以下信息:(i)研究设计,(ii) CVS中报告的hpv特异性抗体浓度(以及相关的血清水平,如果提供的话),(iii) CVS收集方法,(iv)使用的免疫测定方法。结果通过系统的检索和筛选,共获得44篇文献。本文讨论了自然感染(26/44)和HPV疫苗接种(18/44)后CVS中存在HPV特异性抗体的证据。许多研究表明,hpv特异性抗体检测在CVS是可变的,但可行的各种收集方法和免疫测定。大多数CVS样本是通过宫颈阴道冲洗或棉芯收集的,抗体的存在主要是通过基于vlp的elisa检测。血清和CVS中疫苗诱导的抗体水平之间的中度至强相关性表明,HPV疫苗产生的抗体可通过宫颈粘膜上皮渗出。结论虽然hpv特异性抗体在CVS中的滴度低于血清样品,但研究表明在CVS中检测hpv特异性抗体是可行的。然而,已发表的观察结果的高度可变性以及缺乏严格统一的、经过良好验证的抗体收集、分离和定量方法,表明需要特定的方法来改进和标准化CVS中hpv特异性抗体的检测。
期刊介绍:
The official Journal of the International Papillomavirus Society Papillomavirus Research (PVR), the Journal of HPV and other Small DNA Tumor Viruses publishes innovative papers related to all aspects of papillomaviruses and other small DNA tumor viruses. The official journal of the International Papillomavirus Society, PVR is an open access publication that aims to bring together virologists, immunologists, epidemiologists and clinicians working in the booming field of HPV and animal papillomaviruses, polyomaviruses and other small DNA tumor viruses and their associated diseases, in order to foster and facilitate interdisciplinary communication. The journal welcomes original research articles, reviews, short communications, opinion articles and regional update reports on papillomaviruses and other tumor viruses in the following sections: a. Biology of papillomaviruses and related viruses from life cycle to cancer b. Epidemiology etiology and natural history studies c. Natural and induced immunity including vaccine research d. Intervention studies and strategies including i. Clinical studies and trials ii. HPV treatments iii. HPV vaccination programs iv. Diagnostics and screening e. Infection and disease prevention, modeling studies f. Guidelines and public health recommendations g. HPV Studies in special populations Regional and local studies on these viruses.