The complete chloroplast genome of Campsis grandiflora (Bignoniaceae)

IF 0.8 Q4 PLANT SCIENCES KOREAN JOURNAL OF PLANT TAXONOMY Pub Date : 2022-09-30 DOI:10.11110/kjpt.2022.52.3.156
Jongsun Park, Hong Xi
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Abstract

Campsis grandiflora (Thunb.) K. Schum is an ornamental species with various useful biological effects. The chloroplast genome of C. grandiflora isolated in Korea is 154,293 bp long (GC ratio: 38.1%) and has four subregions: 84,121 bp of large single-copy (36.2%) and 18,521 bp of small single-copy (30.0%) regions are separated by 24,332 bp of inverted repeat (42.9%) regions including 132 genes (87 protein-coding genes, eight rRNAs, and 37 tRNAs). One single-nucleotide polymorphism and five insertion and deletion (INDEL) regions (40-bp in total) were identified, indicating a low level of intraspecific variation in the chloroplast genome. All five INDEL regions were linked to the repetitive sequences. Seventy-two normal simple sequence repeats (SSRs) and 47 extended SSRs were identified to develop molecular markers. The phylogenetic trees of 29 representative Bignoniaceae chloroplast genomes indicate that the tribe-level phylogenic relationship is congruent with the findings of previous studies.
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桔梗叶绿体全基因组研究
大花Campsis grandiflora(Thunb.)K.Schum是一种具有多种生物学效应的观赏植物。在韩国分离的大花C.grandiflora叶绿体基因组长154293bp(GC比率:38.1%),有四个亚区:84121bp的大单拷贝(36.2%)和18521bp的小单拷贝(30.0%)区域被24332bp的反向重复(42.9%)区域分隔开,其中包括132个基因(87个蛋白质编码基因、8个rRNA和37个tRNA)。鉴定出一个单核苷酸多态性和五个插入和缺失(INDEL)区(共40个bp),表明叶绿体基因组的种内变异水平较低。所有五个INDEL区域都与重复序列相连。72个正常简单序列重复序列(ssr)和47个扩展ssr被鉴定为开发分子标记。通过对29个具有代表性的大花科叶绿体基因组的系统发育树分析,表明其部落级系统发育关系与前人的研究结果一致。
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来源期刊
KOREAN JOURNAL OF PLANT TAXONOMY
KOREAN JOURNAL OF PLANT TAXONOMY Agricultural and Biological Sciences-Plant Science
CiteScore
1.50
自引率
40.00%
发文量
23
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