Metabolic Analysis of Indole-degradated Metabolites in Two Acinetobacter spp.

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Applied Biochemistry and Microbiology Pub Date : 2023-08-12 DOI:10.1134/S0003683823040154

T. A. Tesso, G. Liu

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Abstract

Indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human, animals and ecological environment. Bacteria play vital role in the degradation and biotransformation of such persistent organic and harmful pollutants. In this experiment, Acinetobacter towneri NTA1-2A and Acinetobacter guillouiae TAT1-6A were used to degrade indole. Indole-degradated metabolites were analyzed by metabolomics analysis of fermentation broth of these strains using LC-MS/MS. The metabolomics analysis showed indole-5-carbonitrile 3-benzoyl (C₁₆H₁₀N₂O), dimethyl sulfoxide (C₂H₆OS), deoxyguanosine (C₁₀H₁₃N₅O₄), Leu (C₆H₁₃NO₂), and N-nitroso-pyrrolidine were indole-degradated metabolites formed by A. towneri NTA1-2A while neurine (C₅H₁₃NO) and norvaline (C₅H₁₁NO₂) are common metabolites of both strains. The two strains' degradation metabolites differed from those reported in other studies, demonstrating the diversity of the distinct microbial strains approach to the metabolism of indolic compounds. Extracellular enzymes released to culture medium by these bacteria were also identified. In conclusion, the two bacterial strains have a potential of converting notorious indole compound to non-toxic intermediates mediated by enzymes. Hence, future study should focus on biochemical pathway and genetic basis for indole-degradated metabolites in the two strains.

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两种不动杆菌吲哚降解代谢物的代谢分析。

吲哚及其衍生物是典型的含氮杂环化合物，因其对人类、动物和生态环境具有毒性、顽固性和致癌性而备受关注。细菌在这些持久性有机和有害污染物的降解和生物转化中起着至关重要的作用。本实验采用towneri不动杆菌NTA1-2A和guillouiae不动杆菌TAT1-6A降解吲哚。采用LC-MS/MS对菌株发酵液进行代谢组学分析，分析吲哚降解代谢物。代谢组学分析表明，吲哚-5-碳腈- 3-苯甲酰(C16H10N2O)、二甲亚砜(C2H6OS)、脱氧鸟苷(C10H13N5O4)、赖氨酸(C6H13NO2)和n -亚硝基吡啶是A. towneri NTA1-2A的吲哚降解代谢产物，而神经氨酸(C5H13NO)和正戊氨酸(C5H11NO2)是这两种菌株的共同代谢产物。这两种菌株的降解代谢物与其他研究报道的不同，表明不同的微生物菌株对吲哚类化合物的代谢方式存在多样性。还鉴定了这些细菌向培养基释放的胞外酶。综上所述，这两种菌株具有将臭名昭著的吲哚化合物转化为酶介导的无毒中间体的潜力。因此，今后的研究重点应放在两菌株吲哚降解代谢物的生化途径和遗传基础上。

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.