Mechanism of the inhibitory effect of acupotomy on chondrocyte apoptosis in KOA rabbits explored via proteomics 运用蛋白质组学技术探讨针刀抗KOA兔软骨细胞凋亡的作用机制

Abstract

Objective

To elucidate the mechanism underlying the inhibitory effect of acupotomy on chondrocyte apoptosis in rabbits with knee osteoarthritis (KOA) through screening, analysis, and verification of apoptosis-related differentially expressed proteins via proteomics.

Methods

Thirty New Zealand white rabbits were randomly divided into the normal, model, and acupotomy groups, with 10 rabbits in each group. The Vidman method was used to prepare KOA models in the model and acupotomy groups. In the acupotomy group, acupotomy was performed at the midpoints of the patellar ligament and medial and lateral collateral ligaments on the left knees once a week for three weeks. Rabbit articular cartilage protein was quantified in each group, and the proteins with differential expression were profiled via proteomics. The apoptosis-related proteins were further screened, and differentially expressed proteins common among the normal, model, and acupotomy groups were collected for cluster analysis. Pathological changes in chondrocytes were observed via HE staining, and chondrocyte apoptosis was observed under an electron microscope. Quantitative real-time PCR (RT-PCR) and western blot were performed to determine the mRNA and protein expression levels, respectively, of the common apoptosis-related proteins in the three groups.

Results

Western blot and RT-PCR showed that the protein and mRNA expression levels of TXN, CAP1 and FABP3 were lower (all P < 0.05), whereas that of LTF, SCIN and IL-10 were higher (all P < 0.01) in the acupotomy group than that in the model group.

Conclusion

The antiapoptotic effect of acupotomy on chondrocytes in KOA rabbits may be achieved by modulating the protein and mRNA expression of differentially expressed apoptosis-related proteins (TXN, CAP1, FABP3, LTS, SCIN, and IL-10).