Concomitant yield optimization of tannase and gallic acid by Bacillus licheniformis KBR6 through submerged fermentation: An industrial approach

Q3 Agricultural and Biological Sciences Acta Biologica Szegediensis Pub Date : 2021-04-10 DOI:10.14232/ABS.2020.2.151-158

P. K. Das Mohapatra, Ishita Biswas, K. C. Mondal, B. Pati

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引用次数: 5

Abstract

The present study is concerned with the evaluation of tannase and gallic acid production eﬃcacy of Bacillus licheniformis KBR6 under different environmental conditions through submerged fermentation. Results have shown that different environmental conditions and mineral sources have differential inﬂuences on tannase and gallic acid production. Highest tannase and gallic acid yield was observed at incubation period of 18 h and 22 h, respectively. At tannic acid concentration of 15 g/l, maximum cell mass (0.75 g/l), cell yield coeﬃcient (0.08 g/g), speciﬁc growth rate (37.5 mg/g/h), tannase yield (16.3 U/g) and speciﬁc tannase production rate (0.80 U/g/h) were observed, however, at higher tannic acid concentration a decrease in tannase yield and production rate were observed, but gallic acid production increased with increasing tannic acid concentration. Additional carbohydrate sources like glucose, fructose, and lactose showed positive inﬂuence on enzyme yield. Among the studied nitrogen sources urea and NH4Cl, and of the phosphate sources KH2PO4 showed favourable effects on cell growth and simultaneous enzyme and gallic acid production. Temperature of 35 °C was found to be optimum for tannase and gallic acid production. Of all the studied metal ions Ca2+, Mg2+ and Na+ showed positive effect whereas, Co2+, Ag2+, Pb2+, Hg2+ showed inhibitory effects.

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地衣芽孢杆菌KBR6深层发酵产单宁酶和没食子酸的工艺优化

本研究旨在通过深层发酵评价地衣芽孢杆菌KBR6在不同环境条件下的单宁酶和没食子酸生产效率。结果表明，不同的环境条件和矿物来源对单宁酶和没食子酸的生产有不同的影响。在培养18小时和22小时时分别观察到最高的单宁酶和没食子酸产量。在单宁酸浓度为15 g/l时，观察到最大细胞质量（0.75 g/l）、细胞产量系数（0.08 g/g）、特定生长速率（37.5 mg/g/h）、单宁酶产量（16.3 U/g）和特定单宁酶生产率（0.80 U/g/h），然而，在较高的单宁酸浓度下，观察到单宁酶产率和生产率下降，但没食子酸的生成量随着单宁酸浓度的增加而增加。其他碳水化合物来源，如葡萄糖、果糖和乳糖，对酶产量有积极影响。在所研究的氮源中，尿素和NH4Cl以及磷酸盐源KH2PO4对细胞生长以及同时产生酶和没食子酸表现出有利的影响。发现35°C的温度是生产单宁酶和没食子酸的最佳温度。在所有研究的金属离子中，Ca2+、Mg2+和Na+表现出积极作用，而Co2+、Ag2+、Pb2+、Hg2+表现出抑制作用。

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来源期刊

Acta Biologica Szegediensis Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

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1.00

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期刊介绍： Acta Biologica Szegediensis (ISSN 1588-385X print form; ISSN 1588-4082 online form), a member of the Acta Universitatis Szegediensis family of scientific journals (ISSN 0563-0592), is published yearly by the University of Szeged. Acta Biologica Szegediensis covers the growth areas of modern biology and publishes original research articles and reviews, involving, but not restricted to, the fields of anatomy, embryology and histology, anthropology, biochemistry, biophysics, biotechnology, botany and plant physiology, all areas of clinical sciences, conservation biology, ecology, genetics, microbiology, molecular biology, neurosciences, paleontology, pharmacology, physiology and pathophysiology, and zoology.