A comparative study on Acorus calamus (Acoraceae) micropropagation and selection of suitable population for cultivation in Iran

Q3 Agricultural and Biological Sciences Acta Biologica Szegediensis Pub Date : 2021-08-21 DOI:10.14232/abs.2021.1.29-34

A. Gholipour, S. Kazemitabar, Hamed Ramzanpour

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引用次数: 1

Abstract

In addition to various medicinal properties, Acorus calamus (sweet ﬂag) is used in health, food, and perfume industries. Since this species is a rare plant in Iran, its propagation and cultivation are of the great importance. The aim of this study was to investigate the effects of different plant growth regulators on micropropaga-tion of this plant and to select the appropriate population. The root, the rhizome and the leaf explants of three populations (Arzefon, Pelesk, and Alandan) were cultured on MS medium supplemented with different concentrations of α-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) for callus induction and plant regeneration. The results showed that only rhizome explant resulted in direct plant regeneration. Among different treatments, the 1 mg/l treatment of BAP and NAA - with the highest mean number of regenerated plants (3.75 ± 0.85), the highest percentage of grown explants (91.6%) and maximum average length of regenerated plants (12.06 ± 0.32 cm) - was the best treatment for regeneration of sweet ﬂag. The highest mean number of root (6.6 ± 0.1) was observed in Alandan population in 1 mg/l treatment of indole-3-butyric acid (IBA). According to the present study, Alandan population is suitable for cultivation purposes in Iran.

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伊朗菖蒲（Acorus calamus）微繁殖及适宜栽培群体选择的比较研究

除了各种药用特性外，菖蒲（甜味剂）还用于健康、食品和香水行业。由于该物种在伊朗是一种稀有植物，其繁殖和栽培具有重要意义。本研究的目的是研究不同植物生长调节剂对该植物微繁殖的影响，并选择合适的群体。将三个群体（Arzefon、Pelesk和Alandan）的根、根茎和叶外植体在添加不同浓度的α-萘乙酸（NAA）和6-苄氨基嘌呤（BAP）的MS培养基上进行愈伤组织诱导和植株再生。结果表明，只有根状茎外植体能直接再生植株。在不同的处理中，BAP和NAA的1mg/l处理——再生植株的平均数量最高（3.75±0.85），外植体的生长率最高（91.6%），再生植株的最大平均长度（12.06±0.32cm）——是甜叶再生的最佳处理。吲哚-3-丁酸（IBA）处理1mg/l时，阿兰丹种群的平均根数最高（6.6±0.1）。根据目前的研究，阿兰丹种群适合在伊朗进行种植。

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Acta Biologica Szegediensis Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

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1.00

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期刊介绍： Acta Biologica Szegediensis (ISSN 1588-385X print form; ISSN 1588-4082 online form), a member of the Acta Universitatis Szegediensis family of scientific journals (ISSN 0563-0592), is published yearly by the University of Szeged. Acta Biologica Szegediensis covers the growth areas of modern biology and publishes original research articles and reviews, involving, but not restricted to, the fields of anatomy, embryology and histology, anthropology, biochemistry, biophysics, biotechnology, botany and plant physiology, all areas of clinical sciences, conservation biology, ecology, genetics, microbiology, molecular biology, neurosciences, paleontology, pharmacology, physiology and pathophysiology, and zoology.