Alleviative Effect of Herbal Extract Containing Phytoconstituents of Bambusa vulgaris and Opuntia ficus-indica against AOM/DSS-Induced Colorectal-Carcinoma-Bearing Mice

Abstract

The study includes preliminary phytochemical screening and assessing alleviative effects of ethanolic extract of Bambusa vulgaris shoots and Opuntia ficus-indica fruits (EEBO) on azoxymethane/dextran sodium sulfate (AOM/DSS)-induced colorectal cancer in rodents. Acute oral toxicity study was performed to find the therapeutic dose of EEBO. In animals, cancer was induced by single injection of AOM 10 mg/kg intraperitoneally followed by supply of three repeated cycles of 2.5% of DSS solution. Then animals were treated with EEBO from third day of experiment and treatment was continued throughout the experimental period. The anticancer effect was assessed by studying body weight (b. wt) changes, macroanatomy, antioxidant level, and microanatomy of colon. The phytochemical evaluation revealed the presence of alkaloid, glycoside, carbohydrate, and flavonoid in bamboo extract, whereas glycoside and flavonoid in cactus extract. The results found that EEBO increases the b. wt and level of antioxidants like sodium dismutase, glutathione, and catalase and meanwhile decreases malondialdehyde. Macroanatomy study indicated a decrease in the numbers of adenoma and inflammatory signs in the treated group when compared with diseased group. Histopathology revealed that the presence of EEBO improved the colon histoarchitecture similar to normal and masked the crypt appearance. Decreased chromosomal aberrations and micronucleus numbers were observed in EEBO treated group. Overall, results suggest that the EEBO 400 mg/kg exhibited highest activity compared with 200 and 100 mg/kg and the effect may be attributed to its antigenotoxic or antioxidant effect.