SINERGISTIC COMBINATION OF ALOE VERA AND KERSEN LEAF EXTRACTS FOR INHIBITION OF THE GROWTH OF Staphylococcus aureus 25923

Jurnal Farmasi Sains dan Praktis Pub Date : 2022-12-01 DOI:10.31603/pharmacy.v8i3.6268

N. Sulistyani, Zikri Almahdi, Z. –, Alfian Syarifuddin

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Abstract

Aloe vera leaf (Aloe vera L.) and cherry leaf (Muntingia calabura L.) contain bioactive compounds that serve as the potential antibacterial agent. The combination treatment of multiple extracts can produce a stronger effect than a single compound. This study aims to determine the synergistic combination of aloe vera leaf extract (EDLB) and cherry leaf extract (EDK) as an antibacterial agent against Staphylococcus aureus ATCC 25923. The extract was obtained by maceration using 96% ethanol. Chemical content in the extract was analyzed by tube test and thin layer chromatography (TLC). Antibacterial activity against S. aureus ATCC 25923 was measured by the checkerboard microdilution method on a 96 well microplate. The effect of the combination of extracts on antibacterial activity was based on the fractional inhibitory concentration index (FICI) value. EDLB and EDK contain flavonoids, tannins, alkaloids, and saponins. The second combination showed a synergistic effect with a FICI value of 0.5 in inhibiting the growth of S. aureus with minimal inhibitory levels in a mixture of 0.625 mg/mL EDLB and 2.5 mg/mL EDK.

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芦荟与克生叶提取物联合抑制金黄色葡萄球菌生长研究

芦荟叶(Aloe vera L.)和樱桃叶(Muntingia calabura L.)含有作为潜在抗菌剂的生物活性化合物。多种提取物的联合治疗可以产生比单一化合物更强的效果。本研究旨在确定芦荟叶提取物(EDLB)和樱桃叶提取物(EDK)联合抑菌对金黄色葡萄球菌ATCC 25923的增效作用。提取液采用96%乙醇浸渍。采用试管法和薄层色谱法对提取液中的化学成分进行了分析。采用棋盘格微量稀释法在96孔微孔板上测定其对金黄色葡萄球菌ATCC 25923的抑菌活性。以分数抑制浓度指数(FICI)值为指标，考察各提取物组合对其抑菌活性的影响。EDLB和EDK含有类黄酮、单宁、生物碱和皂苷。第二种组合对金黄色葡萄球菌的生长抑制具有增效作用，其FICI值为0.5，在0.625 mg/mL EDLB和2.5 mg/mL EDK的混合物中抑制水平最低。

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Jurnal Farmasi Sains dan Praktis

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