RP-HPLC Method for the Simultaneous Estimation of Eight Cardiovascular Drugs

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Chromatographia Pub Date : 2013-11-12 DOI:10.1007/s10337-013-2598-0

Chiguru Vishnuvardhan, P. Radhakrishnanand, Sameer G. Navalgund, Krishnam Raju Atcha, N. Satheeshkumar

{"title":"RP-HPLC Method for the Simultaneous Estimation of Eight Cardiovascular Drugs","authors":"Chiguru Vishnuvardhan, P. Radhakrishnanand, Sameer G. Navalgund, Krishnam Raju Atcha, N. Satheeshkumar","doi":"10.1007/s10337-013-2598-0","DOIUrl":null,"url":null,"abstract":"<p>The present study describes a convenient method for the separation and simultaneous determination of eight drugs used in cardiovascular diseases, viz., atenolol (ATN), lisinopril (LISI), hydrochlorothiazide (HCTZ), enalapril maleate (ENA), amlodipine besylate (AMLO), losartan potassium (LOSA), valsartan (VAL) and atorvastatin calcium (ATOR) in pharmaceutical formulations. A ZORBAX Rx-C8 column (250?×?4.6?mm, 5?μm particle size) was used with mobile phase consisting of acetonitrile, 10?mM dipotassium hydrogen phosphate buffer (pH 2.2 adjusted with orthophosphoric acid) using a gradient program and quantitative evaluation was performed at 210?nm with a flow rate of 1.0?mL?min<sup>?1</sup>. Suitability of this method for the quantitative determination of the drugs was proved by validation in accordance with International Conference on Harmonization guidelines. The method is selective, precise, robust, accurate and can be used for routine analysis of 15 combination pharmaceutical formulations in quality control.</p>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"77 3-4","pages":"265 - 275"},"PeriodicalIF":1.3000,"publicationDate":"2013-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10337-013-2598-0","citationCount":"17","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromatographia","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s10337-013-2598-0","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 17

Abstract

The present study describes a convenient method for the separation and simultaneous determination of eight drugs used in cardiovascular diseases, viz., atenolol (ATN), lisinopril (LISI), hydrochlorothiazide (HCTZ), enalapril maleate (ENA), amlodipine besylate (AMLO), losartan potassium (LOSA), valsartan (VAL) and atorvastatin calcium (ATOR) in pharmaceutical formulations. A ZORBAX Rx-C8 column (250?×?4.6?mm, 5?μm particle size) was used with mobile phase consisting of acetonitrile, 10?mM dipotassium hydrogen phosphate buffer (pH 2.2 adjusted with orthophosphoric acid) using a gradient program and quantitative evaluation was performed at 210?nm with a flow rate of 1.0?mL?min^?1. Suitability of this method for the quantitative determination of the drugs was proved by validation in accordance with International Conference on Harmonization guidelines. The method is selective, precise, robust, accurate and can be used for routine analysis of 15 combination pharmaceutical formulations in quality control.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

反相高效液相色谱法同时测定8种心血管药物的含量

本研究建立了一种简便的方法，用于同时测定制剂中阿替洛尔(ATN)、莱诺普利(LISI)、氢氯噻嗪(HCTZ)、马来酸依那普利(ENA)、苯磺酸氨氯地平(AMLO)、氯沙坦钾(LOSA)、缬沙坦(VAL)和阿托伐他汀钙(ATOR)等8种心血管疾病用药。ZORBAX Rx-C8色谱柱(250 × 4.6?毫米,5 ?μm粒径)，流动相为乙腈，10?mM磷酸氢二钾缓冲液(pH 2.2用正磷酸调节)使用梯度程序和定量评价在210?nm，流速1.0 mL / min。根据国际协调会议的指导方针，通过验证证明了该方法用于药物定量测定的适用性。该方法选择性好，精密度高，稳健性好，准确度高，可用于15种复方制剂的常规分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.