Physiological and transcriptomic analysis of antioxidant mechanisms in sweet sorghum seedling leaves in response to single and combined drought and salinity stress

Abstract

ABSTRACT The effects of drought, salinity, and combined stress on ROS and ROS metabolic physiology and transcriptomics in sweet sorghum seedling leaves were evaluated. The results showed that drought stress had little effect on photosynthesis, while the SOD activity, CAT activity, and the expression of their related genes were elevated in leaves, but no excessive accumulation of O2 -, H2O2, or -OH was observed. Under salinity stress, photosynthesis was inhibited, the O2 -, H2O2 and -OH contents increased significantly, and the SOD, POD, CAT activities and the expression of their related genes in leaves were elevated. Under combined stress, photosynthesis was significantly inhibited, the highest accumulation of O2 -, H2O2 and -OH contents occurred, and the SOD and POD activities and the expression of related genes in leaves were significantly increased, but the CAT was significantly decreased. These results collectively indicate that oxidative damage to sweet sorghum seedling leaves was higher with combined stress than with either drought or salinity stress alone. Under combined stress the SOD and POD activities were increased, but the CAT activity in the AsA-GSH cycle was severely reduced, demonstrating that antioxidant mechanisms in seedlings did not play a normal protective role, leaving the plants severely damaged by oxidative stress. Abbreviations: AsA: Ascorbic acid; APX: Ascorbate peroxidase; CAT: Catalase; Chl a: Chlorophyll a; Chl b: Chlorophyll b; Ci: Intercellular CO2 concentration; DHAR: Dehydroascorbate reductase; DEGs: Differentially expressed genes; DW: Dry weight; ETR: Photosynthetic electron transport rate; FW: Fresh weight; Fv/Fm: Original light energy conversion efficiency; GPX: Glutathione peroxidase; GR: Glutathione reductase; Gs: Stomatal conductance; GSH: Oxidized glutathione; H2O2: Hydrogen peroxide; MDA: Malondialdehyde; MDHAR: Monodehydroascorbate reductase; O2-: Superoxide anion; PCA: Principal component analysis; PCD: Programed cell death; PEG-6000: Polyethylene glycol 6000; POD: Peroxidase; Pn: Photosynthetic rate; Pro: Proline; qRT–PCR: Quantitative real-time PCR; RNA-seq: RNA Sequencing; ROS: Reactive oxygen species; RWC: Relative water content; RMP: Relative membrane permeability; SOD: Superoxide dismutase; SW: Saturation weight; Tr: Transpiration rate; ΦPSII: Actual photochemical efficiency; -OH: Hydroxyl radical