Valorization of citrus sinensis wastes for the production of orange essential oil: identification of chemical composition and antibacterial activity

L. Seid, D. Lakhdari, O. Belgherbi, Ines Laourari, Nasrin Golzadeh, D. Chouder
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Abstract

The objective of this study was to extract, characterize, and examine the antibacterial properties of orange essential oil obtained from citrus sinensis peels, using hydro-distillation method. Various components of the orange oil were characterized by FTIR, UV-Vis and GC- MS spectroscopic techniques. The extracted essential oil was conformed to AFNOR standards, and had a maximum yield of 3.4% (w/w). The obtained results showed that limonene was the major bioactive components of the orange essential oil (97.14%). The essential orange oil and its dilutions in DMSO showed an excellent antibacterial activity against Staphylococcus aureus (gram-positive bacteria) with a diameter of inhibition zone superior to 40 mm. However, it was ineffective antibacterial activity against Escherichia coli and Pseudomonas aerogenes (gram negative bacteria).
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利用柑桔废弃物生产柑桔精油:化学成分及抗菌活性鉴定
本研究采用水蒸气蒸馏法对柑桔果皮中提取的柑桔精油进行提取、表征并考察其抑菌性能。采用FTIR、UV-Vis、GC- MS等光谱技术对橙油的各种成分进行了表征。提取的精油符合AFNOR标准,最大得率为3.4% (w/w)。结果表明,柠檬烯是橙精油的主要生物活性成分(97.14%)。橙精油及其在DMSO中的稀释液对金黄色葡萄球菌(革兰氏阳性菌)具有良好的抑菌活性,抑菌带直径优于40 mm。但对大肠杆菌和产气假单胞菌(革兰氏阴性菌)的抑菌活性不明显。
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