{"title":"Glutathione S -transferase (M1 and T1) and angiotensin-converting enzyme gene polymorphisms and chronic kidney disease in Bangladeshi population","authors":"Jakaria Shawon , Md. Mostafijur Rahman , Zabun Nahar , Yearul Kabir","doi":"10.1016/j.mgene.2021.100981","DOIUrl":null,"url":null,"abstract":"<div><p>Chronic kidney disease (CKD) is a worldwide public health problem that affects a huge number of individuals and documented as a global public health problem. This study was conducted to uncover the association of gene polymorphism of Glutathione S -transferase M1 (GSTM1, rs366631), Glutathione S -transferase T1 (GSTT1, rs17856199), Angiotensin converting enzyme- Insertion/Deletion (ACE-I/D, rs4646994), and Cytochrome P450 Family 11 Subfamily B Member 2 − 344 T/C (CYP11B2 − 344 T/C, rs1799998) with the risk of development of CKD in Bangladeshi population. Blood samples were drawn from 355 participants (175 CKD patients and 180 healthy controls) by an expert phlebotomist. Different techniques like allele-specific multiplex PCR (Polymerase chain reaction), allele-specific PCR, and PCR-RFLP (Polymerase chain reaction-restriction fragment length polymorphism) were used for the genetic polymorphism analysis. Significant associations were evident for GSTT1 null genotype (OR = 2.45; 95% CI = 1.56–3.82; <em>p</em> < 0.001), combined GSTM1-GSTT1 null genotype (OR = 4.16; 95% CI = 1.99–8.64; <em>p</em> < 0.001) and homozygous mutant variant (DD) of ACE- I/D gene (OR = 4.60; 95% CI = 1.77–12.00; <em>p</em> < 0.01). Homozygous mutant variant (DD) of ACE- I/D polymorphism was found to be more prevalent in the male CKD subjects. It is apparent from our findings that the null genotype of GSTT1, combined GSTM1-GSTT1 null genotype, and homozygous mutant variant (DD) of ACE- I/D could be associated with CKD susceptibility.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"30 ","pages":"Article 100981"},"PeriodicalIF":0.8000,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214540021001328/pdfft?md5=ebfa6600d3cbb9d92a4afeffc31efb3b&pid=1-s2.0-S2214540021001328-main.pdf","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Meta Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214540021001328","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 1
Abstract
Chronic kidney disease (CKD) is a worldwide public health problem that affects a huge number of individuals and documented as a global public health problem. This study was conducted to uncover the association of gene polymorphism of Glutathione S -transferase M1 (GSTM1, rs366631), Glutathione S -transferase T1 (GSTT1, rs17856199), Angiotensin converting enzyme- Insertion/Deletion (ACE-I/D, rs4646994), and Cytochrome P450 Family 11 Subfamily B Member 2 − 344 T/C (CYP11B2 − 344 T/C, rs1799998) with the risk of development of CKD in Bangladeshi population. Blood samples were drawn from 355 participants (175 CKD patients and 180 healthy controls) by an expert phlebotomist. Different techniques like allele-specific multiplex PCR (Polymerase chain reaction), allele-specific PCR, and PCR-RFLP (Polymerase chain reaction-restriction fragment length polymorphism) were used for the genetic polymorphism analysis. Significant associations were evident for GSTT1 null genotype (OR = 2.45; 95% CI = 1.56–3.82; p < 0.001), combined GSTM1-GSTT1 null genotype (OR = 4.16; 95% CI = 1.99–8.64; p < 0.001) and homozygous mutant variant (DD) of ACE- I/D gene (OR = 4.60; 95% CI = 1.77–12.00; p < 0.01). Homozygous mutant variant (DD) of ACE- I/D polymorphism was found to be more prevalent in the male CKD subjects. It is apparent from our findings that the null genotype of GSTT1, combined GSTM1-GSTT1 null genotype, and homozygous mutant variant (DD) of ACE- I/D could be associated with CKD susceptibility.
Meta GeneBiochemistry, Genetics and Molecular Biology-Genetics
CiteScore
1.10
自引率
0.00%
发文量
20
期刊介绍:
Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.