Identification of the binding site for plasminogen kringle 5 in the ?-chain of fibrin(ogen) D-fragment

Q4 Biochemistry, Genetics and Molecular Biology Ukrainian Biochemical Journal Pub Date : 2020-08-13 DOI:10.15407/ubj92.03.046
L. G. Kapustianenko, T. Grinenko, A. Rebriev, O. Yusova, A. Tykhomyrov
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Abstract

The interaction of the fifth kringle of Glu-plasminogen with fibrin triggers activation and initiation of fibrinolysis, yet the site on fibrin that binds kringle 5 remains unknown. The aim of our work was to determine an amino acid sequence in the D-fragment of fibrin(ogen) molecule, which is complementary to the lysine-binding site (LBS) in kringle 5. We studied the interaction between kringle 5 of plasminogen with polypeptide chains of the D-fragments of fibrin and cyanogen bromide fragments FCB-2 and t-NDSK and showed that kringle 5 bound specifically to αand γ-chains of the D-fragment and the α-chain of FCB-2. Tryptic peptides of D-fragment α-chain were obtained, separated by their ability to bind with the immobilized kringle 5, and then all studied peptides were characterized by MALDI-TOF analysis. The critical amino acid residues of the α-chain of D-fragment, which provide its interaction with kringle 5, turned out to be α171Arg and/or α176Lys. The binding site of Glu-plasminogen complementary to the LBS of kringle 5 is located within Аα168Ala−183Lys, a sequence in a weakly structured loop between two supercoils in the α-chain of the Dfragment of the fibrin(ogen) molecule.
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纤维蛋白(原)d片段?-链中纤溶酶原kringle 5结合位点的鉴定
葡萄糖-纤溶酶原第5个kringle与纤维蛋白的相互作用触发了纤维蛋白溶解的激活和开始,但纤维蛋白上与kringle 5结合的位点尚不清楚。我们的工作目的是确定纤维蛋白(原)分子d片段的氨基酸序列,该序列与kringle 5中赖氨酸结合位点(LBS)互补。我们研究了纤溶酶原kringle 5与纤维蛋白d片段多肽链和溴化氰片段FCB-2和t-NDSK的相互作用,发现kringle 5特异性结合d片段α和γ链和FCB-2 α-链。获得d片段α-链的色氨酸肽,通过其与固定kringle 5的结合能力进行分离,然后用MALDI-TOF分析对所研究的肽进行表征。d片段α-链上与kringle 5相互作用的关键氨基酸残基为α171Arg和/或α176Lys。与kringle 5的LBS互补的glu -纤溶酶原的结合位点位于Аα168Ala−183Lys内,这是纤维蛋白(原)分子d片段α-链两个超线圈之间的弱结构环中的一个序列。
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来源期刊
Ukrainian Biochemical Journal
Ukrainian Biochemical Journal Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
1.20
自引率
0.00%
发文量
37
审稿时长
16 weeks
期刊介绍: The Ukrainian Biochemical Journal publishes original research papers, reviews and brief notes; papers on research methods and techniques; articles on the history of biochemistry, its development and prominent figures; discussion articles; book reviews; chronicles; etc. The journal scope includes not only biochemistry but also related sciences, such as cellular and molecular biology, bioorganic chemistry, biophysics, pharmacology, genetics, and medicine (medical biochemistry et al.) – insofar as the studies use biochemical methods and discuss biochemical findings.
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