{"title":"Insight into Fluorescence Imaging and Bioorthogonal Reactions in Biological Analysis","authors":"Yuexiang Ma, Qinhua Chen, Xiaoyan Pan, Jie Zhang","doi":"10.1007/s41061-020-00323-5","DOIUrl":null,"url":null,"abstract":"<p>Fluorescence imaging is an important method in the field of biomedicine. Fluorescence imaging is nondestructive, has high efficiency and sensitivity, high resolution and allows real-time dynamic monitoring of living cells. However, it also has some disadvantages, such as high background signals and low selectivity. Bioorthogonal reactions, with the advantages of being both nondestructive and effective, are used to trace and analyze biological interactions in vivo. This review focuses on recent progress in understanding the mechanism of action of fluorescence probes.</p>","PeriodicalId":54344,"journal":{"name":"Topics in Current Chemistry","volume":null,"pages":null},"PeriodicalIF":7.1000,"publicationDate":"2021-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s41061-020-00323-5","citationCount":"18","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Topics in Current Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s41061-020-00323-5","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 18
Abstract
Fluorescence imaging is an important method in the field of biomedicine. Fluorescence imaging is nondestructive, has high efficiency and sensitivity, high resolution and allows real-time dynamic monitoring of living cells. However, it also has some disadvantages, such as high background signals and low selectivity. Bioorthogonal reactions, with the advantages of being both nondestructive and effective, are used to trace and analyze biological interactions in vivo. This review focuses on recent progress in understanding the mechanism of action of fluorescence probes.
期刊介绍：
Topics in Current Chemistry is a journal that presents critical reviews of present and future trends in modern chemical research. It covers all areas of chemical science, including interactions with related disciplines like biology, medicine, physics, and materials science. The articles in this journal are organized into thematic collections, offering a comprehensive perspective on emerging research to non-specialist readers in academia or industry. Each review article focuses on one aspect of the topic and provides a critical survey, placing it in the context of the collection. Selected examples highlight significant developments from the past 5 to 10 years. Instead of providing an exhaustive summary or extensive data, the articles concentrate on methodological thinking. This approach allows non-specialist readers to understand the information fully and presents the potential prospects for future developments.
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