EVALUATION OF THE CRYSTALLINITY OF BACTERIAL CELLULOSE PRODUCED FROM PINEAPPLE WASTE SOLUTION BY USING ACETOBACTER XYLINUM

Q4 Earth and Planetary Sciences ASEAN Engineering Journal Pub Date : 2023-05-31 DOI:10.11113/aej.v13.18868
Thi-Tuyet-Ngan Pham, Thi Tuong An Tran
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引用次数: 1

Abstract

Bacterial cellulose (BC) is a fermentative product of Acetobacter xylinum characterized by high purity and crystallinity of up to 80%. Due to its excellent physical and mechanical properties, bacterial cellulose is increasingly interested in research, especially in the study of applying BC in different fields. Although it is a potential direction, the large-scale production of BC still has certain limitations, mainly the fermentation medium's high cost. Therefore, this study used pineapple waste as a carbon source for BC fermentation. After investigating the influence of fermentation factors on BC yield, this study focused on evaluating the crystallinity of BC under different fermentation conditions. The X-ray diffraction technique was used to determine the crystallinity, while Scanning Electron Microscopy was used to assess the differentiation of the BC structure. The study results showed that, at different fermentation conditions of temperature (25–35°C), time (5–10 days), and bacterial concentration (5–15%), the bacterial cellulose crystallinity was significantly different and in the range of 40.6 % to 83.4 %. The optimum crystallinity of BC was recorded when the experiment was set up at the fermentation temperature of 30°C, 13 days of fermentation time, and bacterial concentration of 14%, with the BC crystallinity being 82.2%.
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木醋杆菌对菠萝废液中细菌纤维素结晶度的评价
细菌纤维素(BC)是木醋杆菌(Acetobacter xylinum)的发酵产物,具有纯度高、结晶度高达80%的特点。细菌纤维素由于其优异的物理力学性能,越来越受到人们的关注,特别是对其在不同领域应用的研究。虽然这是一个有潜力的方向,但大规模生产BC仍有一定的局限性,主要是发酵培养基成本高。因此,本研究利用菠萝废弃物作为BC发酵的碳源。在考察了发酵因素对BC产率的影响后,本研究重点考察了不同发酵条件下BC的结晶度。用x射线衍射技术测定结晶度,用扫描电镜观察BC结构的分化。研究结果表明,在温度(25-35℃)、时间(5-10天)和细菌浓度(5-15%)不同的发酵条件下,细菌纤维素结晶度差异显著,在40.6% ~ 83.4%之间。在发酵温度为30℃,发酵时间为13 d,细菌浓度为14%的条件下,BC的最佳结晶度为82.2%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ASEAN Engineering Journal
ASEAN Engineering Journal Engineering-Engineering (all)
CiteScore
0.60
自引率
0.00%
发文量
75
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