Effects of Feeding OmniGen-AF® during Superovulation on in Vitro Development of Embryos Recovered from Donor Beef Cows

A. Snider, D. Mclean, A. Menino
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Abstract

Embryo quality is crucial when selecting embryos for transfer. Variation in quality may be attributed to poor oocytes, semen, stress, inflammation, and potential immune system dysregulation. OmniGen-AF® (OG) feeding supports immune system function and animal health. Our laboratory recently reported lower percent degenerate embryos recovered and increased plasma progesterone in beef cattle donors fed OG during superovulation. In vitro development of embryos recovered from donor cows fed OG prior to collection is presented here. Embryos were recovered from 24 beef cows assigned to four treatment groups: 0 g OG/hd/d and 200 mg Folltropinr 0 g OG/hd/d and 400 mg FSH (0/400), 56 g OG/hd/d, 200 mg FSH (56/200) and 56 g OG/hd/d and 400 mg FSH (56/400). Good to excellent quality early blastocysts were cultured for 8 d. and development through hatching, embryonic volume and plasminogen activator (PA) production were quantified. The complete protocol was repeated 90 - 120 d later as Replicate 2. Optimal development was observed by embryos recovered from 0/200 cows where percent blastocysts hatching was greater (P compared to 56/200 and 0/400 cows and embryonic volume was greatest (P was similar (P > 0.10) to 56/400 cows and embryos recovered from 56/400 cows in Replicate 1 produced more (P in vitro embryo development similar to that observed for 0/200 cows.
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超排卵期饲喂OmniGen-AF®对供体肉牛体外再生胚胎发育的影响
在选择胚胎进行移植时,胚胎质量至关重要。质量的变化可能归因于卵母细胞、精液、压力、炎症和潜在的免疫系统失调。OmniGen-AF®(OG)饲料支持免疫系统功能和动物健康。我们的实验室最近报道了在超排卵期间饲喂OG的肉牛供体的退化胚胎恢复率较低,血浆黄体酮增加。在收集之前,从饲喂OG的供体奶牛中恢复的胚胎的体外发育在这里提出。选取24头肉牛,将胚胎分为4个处理组:0 g OG/hd/d + 200 mg Folltropinr 0 g OG/hd/d + 400 mg FSH(0/400)、56 g OG/hd/d、200 mg FSH(56/200)和56 g OG/hd/d + 400 mg FSH(56/400)。将优质至优良的早期囊胚培养8 d,通过孵化发育,定量测定胚胎体积和纤溶酶原激活物(PA)的产量。90 - 120d后重复完整的方案作为复制2。0/200奶牛回收的胚胎发育最佳,囊胚孵化率高于56/200和0/400奶牛(P < 0.05),胚胎体积最大(P < 0.05),与56/400奶牛相似,重复1中56/400奶牛回收的胚胎体外胚胎发育率与0/200奶牛相似。
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