Molecular characterization and expression studiesof Eucalyptus globulus stress-responsive gene DHN-10

Abstract

Drought, salinity, and frost result in dehydration of plant cells. The dehydration signals in plants trigger the synthesis of dehydration-induced cellular proteins (dehydrins) which were first observed in maize and barley in 1989 as reported in Yao et al. (2005) and Ali et al. (2014, 2016). Dehydrins belong to a multi-protein family called late embryogenesis abundant (LEA) proteins group-2 (Puhakainen et al. 2004, Yang et al. 2012). The dehydrins are located in the cytoplasm, nucleus, mitochondria, and chloroplast (Xu et al. 2005). Further, dehydrins have been found in the endoplasmic reticulum, plasma membrane, and tonoplasts (Close et al. 1993, Close 1996). The extensive accumulation of dehydrins has been observed in the plant embryos during later developmental stages, just like other LEA proteins (Hanin et al. 2011). The dehydrins accumulate extensively in all vegetative tissues when plants are subjected to environmental stresses that may cause the dehydration of cells like osmotic stress, drought, salinity, and heat (Hanin et al. 2011). The dehydrins are hydrophilic and thermostable