Modulative effect of Physalis alkekengi on both gut bacterial and fungal micro-ecosystem

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL Chinese Herbal Medicines Pub Date : 2023-10-01 DOI:10.1016/j.chmed.2023.02.003
Yanan Yang , Xiaohui Zhao , Yong Xie , Chongming Wu
{"title":"Modulative effect of Physalis alkekengi on both gut bacterial and fungal micro-ecosystem","authors":"Yanan Yang ,&nbsp;Xiaohui Zhao ,&nbsp;Yong Xie ,&nbsp;Chongming Wu","doi":"10.1016/j.chmed.2023.02.003","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. <em>Physalis alkekengi</em> (PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effects of PAL have been verified and gut bacteria are implied in its therapeutic actions. However, the detailed modulation of PAL on gut bacterial species and on gut fungi remains largely unknown. We, therefore, designed a preliminary experiment in normal mice to reveal the modulation effect of PAL on both gut bacteria and fungi, and explore the interaction between them.</p></div><div><h3>Methods</h3><p>Herein, the aqueous extract of PAL was orally administrated to normal C57BL/6 mice for four weeks. The full-length 16S rRNA and ITS1/2 gene sequencing were explored to detect the taxa of gut bacteria and gut fungi after PAL treatment, respectively.</p></div><div><h3>Results</h3><p>Oral administration of PAL notably enriched anti-inflammatory bacterial species such as <em>Duncaniella</em> spp. and <em>Kineothrix alysoides</em>, whereas decreased pro-inflammatory species such as <em>Mucispirillum schaedleri</em>. Simultaneously, PAL increased the abundance of gut fungi <em>Aspergillus ochraceus</em>, <em>Cladosporium</em> sp. and <em>Alternaria</em> sp., and decreased <em>Penicillium janthinellum</em>. Correlation network analysis identified two co-existing microbial groups (groups 1 and 2) that were negatively associated with each other. The group 1 comprised PAL-enriched bacteria and fungi, while group 2 was mainly normal chow-enriched bacteria and fungi. In group 1, <em>Antrodia monomitica</em>, <em>Aspergillus clavatus</em>, <em>Mortierella kuhlmanii</em> and <em>Sarcinomyces</em> sp. MA 4787 were positively correlated with <em>Bifidobacterium globosum</em>, <em>Romboutsia ilealis</em> and so on. In group 2, <em>Chaetomium subspirilliferum</em>, <em>Septoria orchidearum</em> and <em>Cephaliophora tropica</em> were positively related to <em>Lactobacillus</em> spp.</p></div><div><h3>Conclusion</h3><p>Altogether, this preliminary study first demonstrated the modulation effect of PAL on both gut bacteria and gut fungi, which may shed light on the elucidation of PAL’s pharmacological mechanism.</p></div>","PeriodicalId":9916,"journal":{"name":"Chinese Herbal Medicines","volume":"15 4","pages":"Pages 564-573"},"PeriodicalIF":4.7000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1674638423000473/pdfft?md5=0a45b635820a35d8842cfb666f287ef0&pid=1-s2.0-S1674638423000473-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Herbal Medicines","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1674638423000473","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0

Abstract

Objective

Gut microbiome is an intricate micro-ecosystem mediating the human health and drug efficacy. Physalis alkekengi (PAL) is an edible and time-honored traditional Chinese medicine. Several pharmacological effects of PAL have been verified and gut bacteria are implied in its therapeutic actions. However, the detailed modulation of PAL on gut bacterial species and on gut fungi remains largely unknown. We, therefore, designed a preliminary experiment in normal mice to reveal the modulation effect of PAL on both gut bacteria and fungi, and explore the interaction between them.

Methods

Herein, the aqueous extract of PAL was orally administrated to normal C57BL/6 mice for four weeks. The full-length 16S rRNA and ITS1/2 gene sequencing were explored to detect the taxa of gut bacteria and gut fungi after PAL treatment, respectively.

Results

Oral administration of PAL notably enriched anti-inflammatory bacterial species such as Duncaniella spp. and Kineothrix alysoides, whereas decreased pro-inflammatory species such as Mucispirillum schaedleri. Simultaneously, PAL increased the abundance of gut fungi Aspergillus ochraceus, Cladosporium sp. and Alternaria sp., and decreased Penicillium janthinellum. Correlation network analysis identified two co-existing microbial groups (groups 1 and 2) that were negatively associated with each other. The group 1 comprised PAL-enriched bacteria and fungi, while group 2 was mainly normal chow-enriched bacteria and fungi. In group 1, Antrodia monomitica, Aspergillus clavatus, Mortierella kuhlmanii and Sarcinomyces sp. MA 4787 were positively correlated with Bifidobacterium globosum, Romboutsia ilealis and so on. In group 2, Chaetomium subspirilliferum, Septoria orchidearum and Cephaliophora tropica were positively related to Lactobacillus spp.

Conclusion

Altogether, this preliminary study first demonstrated the modulation effect of PAL on both gut bacteria and gut fungi, which may shed light on the elucidation of PAL’s pharmacological mechanism.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
酸浆对肠道细菌和真菌微生态系统的调节作用
目的肠道微生物群是调节人体健康和药物疗效的复杂微生态系统。白Physalis alkekengi (PAL)是一种历史悠久的食用中药。PAL的几种药理作用已得到证实,其治疗作用与肠道细菌有关。然而,PAL对肠道细菌种类和肠道真菌的详细调节在很大程度上仍然未知。因此,我们设计了正常小鼠的初步实验,以揭示PAL对肠道细菌和真菌的调节作用,并探讨它们之间的相互作用。方法以正常C57BL/6小鼠为实验对象,口服PAL水提物4周。利用16S rRNA全长和ITS1/2基因测序分别检测PAL处理后肠道细菌和肠道真菌的分类群。结果口服PAL可显著增加Duncaniella spp和Kineothrix alysoides等抗炎细菌种类,而减少Mucispirillum schaedleri等促炎细菌种类。同时,PAL增加了肠道真菌赭曲霉、枝孢霉和Alternaria sp.的丰度,减少了紫青霉。相关网络分析发现两个共存的微生物群(组1和组2)彼此负相关。第1组以富含pal的细菌和真菌为主,第2组以富含正常食物的细菌和真菌为主。在1组中,单歧Antrodia、clavatus Aspergillus、kuhlmanii Mortierella、Sarcinomyces sp. MA 4787与globosum双歧杆菌、Romboutsia ilealis等呈显著正相关。在第2组中,毛藻(Chaetomium subspirilliferum)、兰花Septoria orchidearum和热带头孢(Cephaliophora tropica)与乳酸菌(Lactobacillus sp .)呈正相关。结论本初步研究首次证实了PAL对肠道细菌和肠道真菌的调节作用,为阐明PAL的药理作用机制提供了线索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
期刊介绍: Chinese Herbal Medicines is intended to disseminate the latest developments and research progress in traditional and herbal medical sciences to researchers, practitioners, academics and administrators worldwide in the field of traditional and herbal medicines. The journal's international coverage ensures that research and progress from all regions of the world are widely included. CHM is a core journal of Chinese science and technology. The journal entered into the ESCI database in 2017, and then was included in PMC, Scopus and other important international search systems. In 2019, CHM was successfully selected for the “China Science and Technology Journal Excellence Action Plan” project, which has markedly improved its international influence and industry popularity. CHM obtained the first impact factor of 3.8 in Journal Citation Reports (JCR) in 2023.
期刊最新文献
DNA metabarcoding uncovers fungal communities in Zingiberis Rhizoma Compound Danshen Dripping Pills combined with isosorbide mononitrate for angina pectoris: A systematic review and a Meta-analysis Metabolomics combined with network pharmacology reveals anti-asthmatic effects of Nepeta bracteata on allergic asthma rats Mechanisms of Shufeng Jiedu Capsule in treating bacterial pneumonia based on network pharmacology and experimental verification Deciphering relationship between depression and microbial molecules based on multi-omics: A case study of Chaigui Granules
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1