EFFECT OF GAMMA IRRADIATION ON Salvia officinalis L. AND Melissa officinalis L. IN VITRO PLANTS

IF 0.6 Q4 AGRONOMY AgroLife Scientific Journal Pub Date : 2023-06-30 DOI:10.17930/agl2023118

A. Radomir, R. Stan, I. Guţă, Mariana Letiția Pandelea, D. Vizitiu, C. Neguț

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Abstract

Salvia officinalis L. (sage) and Melissa officinalis L. (lemon balm) are two valuable medicinal plants from Lamiaceae family. Considering the therapeutic potential of sage and lemon balm extracts, there is currently great interest to increase the production of biological material and the synthesis of bioactive compounds by different methods. Both micropropagation and gamma irradiation represent efficient methods of stimulating the synthesis of bioactive compounds in plants. In order to produce biological material, it is important to establish the doses of gamma radiation that do not have a phytotoxic effect on plants. The aim of the present study was to evaluate the effect of different doses of gamma irradiation (100, 300, and 500 Gy) on sage and lemon balm by quantifying of some biochemical compounds (assimilatory pigments, soluble carbohydrates, and total polyphenols) in in vitro plants. The results obtained revealed that high doses of gamma radiation have phytotoxic effect on in vitro raised plants. However, micropropagation is an efficient method to produce high quality biological material, source for obtaining extracts with therapeutic potential.

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γ辐照对离体植物丹参和梅丽莎的影响

鼠尾草（Salvia officinalis L.）和梅丽莎·officinals L。考虑到鼠尾草和柠檬风油精提取物的治疗潜力，目前人们对通过不同方法增加生物材料的生产和生物活性化合物的合成非常感兴趣。微繁殖和γ辐射都是刺激植物中生物活性化合物合成的有效方法。为了生产生物材料，重要的是确定对植物没有植物毒性影响的伽马辐射剂量。本研究的目的是通过量化体外植物中的一些生物化学化合物（同化色素、可溶性碳水化合物和总多酚）来评估不同剂量的伽马辐射（100、300和500 Gy）对鼠尾草和柠檬风油精的影响。结果表明，高剂量γ射线对体外培养的植物具有植物毒性作用。然而，微繁殖是生产高质量生物材料的有效方法，也是获得具有治疗潜力的提取物的来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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