Molecular epidemiology of group A rotavirus in children with diarrhea in Xiamen

中华传染病杂志 Pub Date : 2019-06-15 DOI:10.3760/CMA.J.ISSN.1000-6680.2019.06.002

Shunqin Wang, Jiancheng Lin, Xiuxiang Xiao, Hai-yan Wu, J. Xu

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Abstract

Objective To investigate the epidemiological characteristics and genotypes of group A rotavirus (RV-A) among inpatients and outpatients children with diarrhea in Xiamen to provide basic data and theoretical basis for prevention and treatment of rotavirus diarrhea. Methods A total of 5 787 fecal samples from children under 10 years old in four hospitals in Xiamen from Jan 2016 to Dec 2017 were detected by immunochromamatoraphy double antibody sandwich assay. Systematic sampling was applied for collection of 98 fecal samples from 1 435 samples with rotavirus positive. Reverse transcription nested PCR was applied for determination of G and P genotypes. Results Among the 5 787 patients, 1 435 specimens were detected to be RV positive (24.8%). Genotyping of 98 rotaviruses showed that G9 (69.4%) was the most predominant, followed by G2 (5.1%), G1 (4.1%) and G3 (1.0%). Twenty cases were undetermined as G type. For P types, P[8]was predominant, accounting for 75.5% and the prevalence of P[4] was 5.1%. Nineteen cases were undetermined as P type. The combination of genotypes were P[8]G9 (64.3%), followed by P[4]G2 (5.1%), P[8]G1 (4.1%) and P[8]G3 (1.0%). Conclusions Rotavirus is the main pathogen among infants and children with diarrhea in Xiamen. P[8]G9 is the most prevalent genotypes. Continuously monitoring RV-A epidemic genotypes is helpful to provide data for local prevention and control of RV-A infection and introduction of rotavirus vaccine. Key words: Rotavirus; Diarrhea; Genotype; Epidemiology

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厦门市腹泻患儿A组轮状病毒分子流行病学分析

目的了解厦门市住院和门诊腹泻患儿中A组轮状病毒(RV-A)的流行病学特征及基因型，为轮状病毒腹泻的防治提供基础数据和理论依据。方法采用免疫层析双抗体夹心法对2016年1月至2017年12月厦门市4家医院10岁以下儿童粪便标本5 787例进行检测。对1 435份轮状病毒阳性粪便标本采用系统抽样方法采集98份粪便标本。采用反转录巢式PCR法测定G和P基因型。结果5 787例患者中，RV阳性标本检出1 435例(24.8%)。98株轮状病毒基因分型结果显示，以G9型(69.4%)为主，其次为G2型(5.1%)、G1型(4.1%)和G3型(1.0%)。20例未确定为G型。P型以P[8]型为主，占75.5%，P[4]型患病率为5.1%。未确定为P型19例。基因型组合为P[8]G9(64.3%)，其次为P[4]G2(5.1%)、P[8]G1(4.1%)和P[8]G3(1.0%)。结论轮状病毒是厦门市婴幼儿腹泻的主要致病菌。p[8]g9是最普遍的基因型。持续监测甲型病毒流行基因型，有助于为当地预防和控制甲型病毒感染和轮状病毒疫苗的引入提供数据。关键词:轮状病毒;腹泻;基因型;流行病学

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中华传染病杂志

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期刊介绍： The Chinese Journal of Infectious Diseases was founded in February 1983. It is an academic journal on infectious diseases supervised by the China Association for Science and Technology, sponsored by the Chinese Medical Association, and hosted by the Shanghai Medical Association. The journal targets infectious disease physicians as its main readers, taking into account physicians of other interdisciplinary disciplines, and timely reports on leading scientific research results and clinical diagnosis and treatment experience in the field of infectious diseases, as well as basic theoretical research that has a guiding role in the clinical practice of infectious diseases and is closely integrated with the actual clinical practice of infectious diseases. Columns include reviews (including editor-in-chief reviews), expert lectures, consensus and guidelines (including interpretations), monographs, short monographs, academic debates, epidemic news, international dynamics, case reports, reviews, lectures, meeting minutes, etc.