Effect of RNA binding protein 6 on proliferation, migration and apoptosis of laryngeal cancer cells

中华实验外科杂志 Pub Date : 2020-01-08 DOI:10.3760/CMA.J.ISSN.1001-9030.2020.01.035

Jiancheng Tan, Xinguo Liu, Jinyan Xing, Yongyuan Tian, Hui-Ping Zhao, Dan Yang, Liang Wang

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Abstract

Objective To investigate the effect of RNA binding protein 6 (RBM6) on the proliferation, migration and apoptosis of laryngeal cancer cells. Methods The expression of RBM6 protein was analyzed by Western blotting in 47 cases of laryngeal cancer and adjacent tissues. RBM6 overexpression cell line and control cell line (RBM6 group and control group) were constructed by RBM6 overexpression lentivirus and control lentivirus respectively. The proliferation of two groups was analyzed by cell counting kit-8 (CCK-8). The migration was analyzed in RBM6 group and control group by scratch test. The apoptosis level in RBM6 group and control group was analyzed by flow cytometry. The growth of tumor cells in vivo in RBM6 group and control group was analyzed by allogeneic tumor transplantation. SPSS 13.0 statistical software was used to analyze the measurement data. Mean±SD was used to express the measurement data, and t-test was used to compare between groups. Results As compared with the level of RBM6 protein in adjacent tissues (1.01±0.23), the expression level of RBM6 protein (0.34±0.12) in laryngeal cancer significantly decreased (t=2.913, P<0.05). As compared with the control group, the cell proliferation ability of RBM6 group significantly decreased (F=3.019, P<0.05). As compared with the control group [(84.18±8.32)%], Scratch healing rate in RBM6 group [(30.11±6.89)%] significantly enhanced (t=4.319, P<0.05). As compared with the control group [(35.61±7.01)%], the apoptosis rate in RBM6 group [(5.23±2.12)%] significantly increased (t=2.192, P<0.05). The results showed that the proliferation rate of cells in the control group was significantly higher than that in the RBM6 group (F=2.908, P<0.05). Conclusion RBM6 can inhibit the proliferation and migration of laryngeal cancer cells, and promote the apoptosis of tumor cells. Key words: RNA binding protein 6; Laryngeal cancer; Proliferation; Migration; Apoptosis

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RNA结合蛋白6对喉癌症细胞增殖、迁移和凋亡的影响

目的探讨RNA结合蛋白6（RBM6）对喉癌症细胞增殖、迁移和凋亡的影响。方法应用蛋白质印迹法检测47例癌症及癌旁组织中RBM6蛋白的表达。用RBM6过表达慢病毒和对照慢病毒分别构建了RBM6过度表达细胞系和对照细胞系（RBM6组和对照组）。用细胞计数试剂盒-8（CCK-8）检测两组细胞增殖情况。通过划痕试验分析RBM6组和对照组的迁移情况。流式细胞仪检测RBM6组和对照组细胞凋亡水平。通过同种异体肿瘤移植分析RBM6组和对照组的肿瘤细胞在体内的生长。采用SPSS 13.0统计软件对测量数据进行分析。平均值±标准差用于表示测量数据，t检验用于组间比较。结果与癌旁组织RBM6蛋白表达水平（1.01±0.23）相比，喉癌症RBM6蛋白的表达水平（0.34±0.12）显著降低（t=2.913，P＜0.05），与对照组相比，RBM6组细胞增殖能力显著降低（F=3.019，P＜0.01），RBM6组抓痕愈合率[（30.11±6.89）%]明显高于对照组[（35.61±7.01）%]（t=4.319，RBM6组细胞凋亡率[（5.23±2.12）%]显著升高（t=2.192，P＜0.05），对照组细胞增殖率显著高于RBM6组（F=2.908，P＜0.01）。关键词：RNA结合蛋白6；癌症；扩散；移民；细胞凋亡

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中华实验外科杂志

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