The Influence of Breed and Type of Extender on the Quality of Bull Semen

Iman Sukirman, E. Sukmawati, S. Rasad, N. Solihati
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引用次数: 2

Abstract

This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.
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品种和膨化剂类型对公牛精液质量的影响
本研究旨在确定添加剂的品种和类型对BIB Lembang奶牛冷冻精液质量的影响。实验研究采用因子随机分组设计(RBD),有两个因素。第一个因素是四个奶牛品种,即Ongole Cross(PO)、Brahman(BR)、Simmental(SM)和Limousin(LM),第二个因素是两种类型的扩展器,即Skim Egg Yolk(SKT)和AndroMed®(and),所有这些都重复了四次。观察到的变量是精子活力百分比和完整质膜(IPM)。使用通用线性模型(IBMSPSS第23版)对获得的所有数据进行分析。结果表明,品种和稀释剂类型之间存在相互作用。不同品种的精液活力差异显著,但完整质膜(MPU)差异不显著。稀释剂的类型对冷冻精液的活力和完整的质膜(MPU)没有显著影响。该品种对BR活力的影响较低,与PO、LM和SM显著不同。稀释剂类型对活力、MPU没有显著影响。结果表明,SKT低于AND,表明品种对完整质膜PO的影响低于BR、LM和SM,稀释剂类型对完整质膜AND的影响低于SKT。冷冻精液中活力降低最低的是使用脱脂蛋黄增稠剂的婆罗门牛。
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发文量
9
审稿时长
4 weeks
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