Initial high throughput proteomic analysis reveals alterations in CD19+ B lymphocyte profile in acute COVID-19 patients

IF 1.1 4区医学 Q4 VIROLOGY Acta virologica Pub Date : 2023-08-22 DOI:10.3389/av.2023.11702

Miroslav Marcin, Ondrej Zahornacký, Mária Kacírová, M. Sulíková, Miloslava Bajer-Ďuráčová, P. Rehulka, P. Bober, J. Fedacko, P. Jarčuška, J. Sabó

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Abstract

The immune response to SARS-CoV-2, the virus responsible for COVID-19, involves intricate interactions between immune cells and viral antigens. CD19+ lymphocytes play a critical role in driving the humoral immune response. In this study, high-throughput proteomic analysis was performed using tandem mass spectrometry to investigate the changes in proteomic profiles of CD19+ whole cell lysates from 6 healthy individuals and 6 acute COVID-19 patients. The volcano plot and heat map showed significant differences in proteomic profiles between these two groups, indicating a distinct molecular signature associated with acute COVID-19. Enrichment analysis, especially over-representation analysis (ORA) using the Reactome database, revealed that proteins involved in neutrophil degranulation and interferon alpha/beta signaling pathways were among the most affected, indicating alterations in key defense processes. These findings, therefore, provide new insights into the molecular mechanisms underlying CD19+ cell responses in acute COVID-19.

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初步高通量蛋白质组学分析揭示急性新冠肺炎患者CD19+B淋巴细胞谱的变化

对导致新冠肺炎的SARS-CoV-2病毒的免疫反应涉及免疫细胞和病毒抗原之间复杂的相互作用。CD19+淋巴细胞在驱动体液免疫反应中起着关键作用。在本研究中，使用串联质谱法进行高通量蛋白质组学分析，以研究6名健康个体和6名急性新冠肺炎患者的CD19+全细胞裂解物的蛋白质组学图谱的变化。火山图和热图显示了这两组之间蛋白质组学图谱的显著差异，表明与急性新冠肺炎相关的独特分子特征。富集分析，特别是使用Reactome数据库的过度表达分析（ORA）显示，参与中性粒细胞脱颗粒和干扰素α/β信号通路的蛋白质受到的影响最大，表明关键防御过程发生了变化。因此，这些发现为急性新冠肺炎中CD19+细胞反应的分子机制提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta virologica 医学-病毒学

CiteScore

3.10

自引率

11.80%

发文量

审稿时长

>12 weeks

期刊介绍： Acta virologica is an international journal of predominantly molecular and cellular virology. Acta virologica aims to publish papers reporting original results of fundamental and applied research mainly on human, animal and plant viruses at cellular and molecular level. As a matter of tradition, also rickettsiae are included. Areas of interest are virus structure and morphology, molecular biology of virus-cell interactions, molecular genetics of viruses, pathogenesis of viral diseases, viral immunology, vaccines, antiviral drugs and viral diagnostics.