Sex-Based Variation of Gene Expression in the Gonads and Fins of Russian Sturgeon (Acipenser gueldenstaedtii)

G. Degani, Akram Hajouj, A. Hurvitz
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引用次数: 3

Abstract

Russian sturgeon (Acipenser gueldenstaedtii) is a primitive freshwater fish and a source of black caviar. The genes involved in sexual determination and differentiation are still unknown and there are no molecular markers for sex identification in this species. Studying the variation of the sex-based differences in genomic sequences and in gene expression in the sturgeon may lead to markers of sex in early stages of development and advances in aquaculture, as well as provide novel insights about the evolution of reproduction, sex determination, and sexual differentiation mechanisms in vertebrates. Previous studies by our and other groups have identified differentially expressed genes in the gonads of adult female and male sturgeon. The current study aimed to test whether these genes were also differentially expressed in non-gonadal tissue, namely fins. We measured by qRT-PCR the mRNA levels of 29 known and novel sex-related genes in the gonads and fins of males (4 years old) and females (7 years old; sexual maturation is earlier in males than in females). Six genes (ATP6, IGFRM, LIA1A, S1A, NPL1A, GAPDH and SOX9) showed higher expression in female fins. However, only ATP6 mRNA levels differed in fins of males and females of the same age (4 years old). These findings underscore the impracticality of sex identification based on gene expression in non-gonadal tissue and the need for genetic sex markers in the Russian sturgeon.
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俄罗斯鲟性腺和鳍基因表达的性别变异
俄罗斯鲟是一种原始淡水鱼,也是黑鱼子酱的来源。参与性别决定和分化的基因仍然未知,并且在该物种中没有用于性别鉴定的分子标记。研究鲟鱼基因组序列和基因表达中基于性别的差异的变化,可能会在水产养殖的发展和进步的早期阶段发现性别标记,并为脊椎动物繁殖、性别决定和性别分化机制的进化提供新的见解。我们和其他研究小组先前的研究已经确定了成年雌性和雄性鲟鱼性腺中差异表达的基因。目前的研究旨在测试这些基因是否在非性腺组织,即鳍中也有差异表达。我们通过qRT-PCR测量了雄性(4岁)和雌性(7岁;雄性的性成熟早于雌性)性腺和鳍中29个已知和新的性相关基因的mRNA水平。6个基因(ATP6、IGFRM、LIA1A、S1A、NPL1A、GAPDH和SOX9)在雌性鳍中表现出较高的表达。然而,在相同年龄(4岁)的雄性和雌性的鳍中,只有ATP6 mRNA水平不同。这些发现强调了基于非性腺组织中基因表达的性别鉴定的不切实际,以及对俄罗斯鲟遗传性别标记的需要。
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