Probing the Production of Intracellular Vesicles Containing Reactive Oxygen and Nitrogen Species by Electrochemical Resistive-pulse Sensing

IF 1.7 Q4 ELECTROCHEMISTRY Electrochemical Society Interface Pub Date : 2022-12-01 DOI:10.1149/2.f07224if
Rui Jia
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Abstract

Reactive oxygen and nitrogen species (ROS/RNS) are known to play an essential role in cell signaling, disease development and progression. The production of ROS/RNS in living cells can be induced by diacylglycerol-lactone (DAG-lactone) through activation of protein kinase C, an important therapeutic target for cancer and other diseases. In a previous report, nano-electrochemistry was performed to evaluate the production of ROS/RNS inside a human breast cell (MCF-10A) treated with DAG-lactone. Simultaneously, the formation of large intracellular vacuoles was observed using a microscope. These results suggest a possibility that the ROS/RNS were stored in the intracellular vacuoles. The experiments carried out during this summer were aimed to elucidate the relationship between the intracellular production of vesicles and ROS/RNS.
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利用电化学电阻脉冲传感探测细胞内含有活性氧和活性氮的囊泡的产生
活性氧和活性氮(ROS/RNS)在细胞信号传导、疾病发生和进展中起着重要作用。活细胞中ROS/RNS的产生可由二酰基甘油内酯(dag -内酯)通过激活蛋白激酶C来诱导,蛋白激酶C是癌症等疾病的重要治疗靶点。在之前的一份报告中,采用纳米电化学方法评估了用dag -内酯处理的人乳腺细胞(MCF-10A)中ROS/RNS的产生。同时,在显微镜下观察到细胞内大液泡的形成。这些结果提示ROS/RNS可能储存在细胞内液泡中。今年夏天进行的实验旨在阐明细胞内囊泡产生与ROS/RNS之间的关系。
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CiteScore
2.10
自引率
5.60%
发文量
62
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