Effect of long non-coding RNA maternally expressed gene 3 on proliferation and invasion capacity of glioma cells through the Wnt/human-catenin signal pathway

中华实验外科杂志 Pub Date : 2020-01-08 DOI:10.3760/CMA.J.ISSN.1001-9030.2020.01.019

Shifeng Yang, Lei Wang, Xiao Ma, Mingqi Yang

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Abstract

Objective To investigate the effect of long non-coding RNA maternally expressed gene 3 (MEG3) on the proliferation and invasion capacity of glioma cells through the Wnt/human-catenin signal pathway. Methods Glioma cells were divided into 3 groups: the blank control group (CON), the gene transfection group (MEG3) and the gene inhibition group (siMEG3). The cells in blank group were left untreated and cultured normally. MEG3 gene was transfected in the gene transfection group, and gene interference was conducted in the gene inhibition group. Protein expression was determined by Western blotting, and gene expression was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR). Results After transfection of MEG3 gene, the apoptosis rate increased to 61.05±2.77 with the prolongation of culture time, which was higher than that of the control group and the gene inhibition group (F=23.543, P<0.05). After MEG3 gene knockout, the apoptosis rate of glioma cells was higher than that of the control group (P<0.05). After 48 hours of transfection, the number of cell migration was 105.36±15.27 (F=33.350, P<0.05). When MEG3 gene was inhibited, the number of cell migration was 989.41±11.06, higher than that of the control group (F=40.667, P<0.05). After MEG3 gene transfection, the number of invasion cells was 251.25±35.85, which was lower than that of the control group and gene knockout group (F=31.167, P<0.05). After MEG3 gene was knocked out, the number of invasion cells was 1 500.00± 84.76, which was higher than that of the blank control group (F=63.762, P<0.05). Compared with the gene inhibition group, the c-Jun gene of the control group and the gene transfection group decreased, and the gene transfection group was lower than the blank control group (F=15.426, P<0.05). Conclusion Long non-coding RNA MEG3 can inhibit the proliferation and invasion of glioma cells through the Wnt/human-catenin signal pathway. Key words: Glioma; Protein; Gene

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长非编码RNA母系表达基因3通过Wnt/人连环蛋白信号通路对胶质瘤细胞增殖和侵袭能力的影响

目的通过Wnt/人连环蛋白信号通路研究长非编码RNA母细胞表达基因3（MEG3）对胶质瘤细胞增殖和侵袭能力的影响。方法将胶质瘤细胞分为3组：空白对照组（CON）、基因转染组（MEG3）和基因抑制组（siMEG3）。空白组细胞未经处理，正常培养。基因转染组转染MEG3基因，基因抑制组进行基因干扰。蛋白质表达通过蛋白质印迹法测定，基因表达通过实时定量逆转录聚合酶链式反应（RT-qPCR）检测。结果转染MEG3基因后，细胞凋亡率随培养时间的延长而增加至61.05±2.77，高于对照组和基因抑制组（F=23.543，P<0.05），细胞迁移数为105.36±15.27（F=33.350，P<0.05）。MEG3基因被抑制时，细胞迁移数989.41±11.06，高于对照组（F=40.667，P<0.05）；MEG3基因转染后，侵袭细胞数251.25±35.85，MEG3基因敲除后，侵袭细胞数为1500.00±84.76，高于空白对照组（F=63.762，P<0.05）。与基因抑制组相比，对照组和基因转染组的c-Jun基因减少，结论长非编码RNA MEG3可通过Wnt/人连环蛋白信号通路抑制胶质瘤细胞的增殖和侵袭。关键词：胶质瘤；蛋白质；基因

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