Inferring population structure and genetic diversity of the invasive alien Nootka lupin in Iceland

IF 1.9 4区 地球科学 Q3 ECOLOGY Polar Research Pub Date : 2021-02-17 DOI:10.33265/POLAR.V40.4536
J. Skorupski, M. Szenejko, Martyna Gruba-Tabaka, P. Śmietana, R. Panicz
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引用次数: 1

Abstract

Polar and subpolar regions are known for their particular vulnerability and sensitivity to the detrimental effects of non-indigenous species, which is well exemplified by the Nootka lupin (Lupinus nootkatensis) spread in Iceland. Since understanding the population and ecological genetics of invasive alien species offers hope for counteracting harmful biological invasions, the objective of the present study was to investigate interspecific variation in L. nootkatensis in Iceland in relation to a native population in Alaska. Moreover, we aimed to assess whether internal transcribed spacer 2 (ITS2) has sufficient phylogenetic applicability for a large-scale screening of the genetic diversity of a non-indigenous population of this species. This study, which is the first attempt to investigate the genetic diversity of the Nootka lupin in Iceland, included plant samples from eight locations in Iceland and one in Alaska. The analyses included genotyping by sequencing of the 417-nucleotide fragment of the 5.8S ribosomal RNA, ITS2 and part of the large subunit ribosomal RNA (GenBank MT026578-MT026580, MT077004). The main findings showed the presence of five previously unexplained single-nucleotide polymorphisms (SNPs); however, their discriminatory power for Icelandic populations was relatively low, since polymorphism information content (PIC) values ranged from 0.0182 to 0.0526, with average heterozygosity 0.0296. Concomitantly, analysis of multilocus genotypes (MLG) revealed sufficient differences in MLGs variants and their frequency to form genotypic patterns unique for Alaskan and Icelandic populations, revealing an internal genetic structure of the studied group. The proposed SNP panel needs to be supplemented with other nuclear and organellar markers.
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冰岛外来入侵木虱种群结构及遗传多样性分析
极地和亚极地以其对非本土物种有害影响的特殊脆弱性和敏感性而闻名,在冰岛传播的Nootka羽扇豆就是一个很好的例子。由于了解外来入侵物种的种群和生态遗传学为抵御有害的生物入侵提供了希望,本研究的目的是研究L。冰岛的nootkatensis与阿拉斯加的土著人口有关。此外,我们旨在评估内部转录间隔区2(ITS2)是否具有足够的系统发育适用性,用于大规模筛选该物种非本土种群的遗传多样性。这项研究首次尝试调查冰岛Nootka羽扇豆的遗传多样性,包括来自冰岛八个地点和阿拉斯加一个地点的植物样本。分析包括通过测序5.8S核糖体RNA的417核苷酸片段、ITS2和部分大亚基核糖体RNA(GenBank MT026578-MT026580,MT077004)进行基因分型。主要研究结果显示存在五种以前无法解释的单核苷酸多态性(SNPs);然而,他们对冰岛人群的辨别力相对较低,因为多态性信息含量(PIC)值在0.0182至0.0526之间,平均杂合度为0.0296。同时,对多点基因型(MLG)的分析揭示了MLG变体及其频率的足够差异,从而形成阿拉斯加和冰岛群体特有的基因型模式,揭示了研究群体的内部遗传结构。提议的SNP小组需要补充其他的细胞核和组织器标记。
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来源期刊
Polar Research
Polar Research 地学-地球科学综合
CiteScore
3.20
自引率
5.30%
发文量
22
审稿时长
>12 weeks
期刊介绍: Since 1982, Polar Research has been the international, peer-reviewed journal of the Norwegian Polar Institute, Norway''s central institution for research, environmental monitoring and mapping of the polar regions. Aiming to promote the exchange of scientific knowledge about the Arctic and Antarctic across disciplinary boundaries, Polar Research serves an international community of researchers and managers. As an open-access journal, Polar Research makes its contents freely available to the general public. Original primary research papers comprise the mainstay of Polar Research. Review articles, brief research notes, letters to the editor and book reviews are also included. Special issues are published from time to time. The scope of Polar Research encompasses research in all scientific disciplines relevant to the polar regions. These include, but are not limited to, the subfields of biology, ecology, geology, oceanography, glaciology and atmospheric science. Submissions from the social sciences and those focusing on polar management and policy issues are welcome. Contributions about Antarctica are particularly encouraged.
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