Detection of A1 and A2 milk in Tiruchirappalli district using TANUVAS A1A2detect kit

IF 0.2 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE INDIAN JOURNAL OF DAIRY SCIENCE Pub Date : 2022-06-22 DOI:10.33785/ijds.2022.v75i03.013
V. Jayalalitha, K. Shibi Thomas, P. R. Richard Jagatheesan
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Abstract

Milk contributes a vital role in nutritional security in most of the countries of the world. Recently a debate is going on worldwide that, A1 milk is harmful and A2 milk is beneficial. Though there was no supporting clinical evidence to claim these, milk is selling in the name of A2 milk for high cost without proper identification in the field level. To address this issue to both consumer and producers, scientists are in a position to rule out the A1 and A2 milk. In this study TANUVAS A1A2 detect kit was used in the A1 or A2 type milk with dried blood or milk spot. 31 samples were collected from farms and individual farmers from different places of Tiruchirappalli district. All the samples were analysed with the ready to use primer based PCRÂ test TANUVAS A1A2 detect kit. This study revealed that 20% of the crossbred cows, 78.95% of the indigenous breed cows, 66.67 % of non descriptive cows and 100 percent of the buffaloes had A2A2 genotype. The results of this study is giving the fact that, milk needs to be checked out with proper test like TANUVAS A1A2 detect before claiming for A2 milk.
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使用TANUVAS a1a2检测试剂盒检测Tiruchirappalli地区A1和A2乳
在世界上大多数国家,牛奶在营养安全方面发挥着至关重要的作用。最近,全世界都在争论A1牛奶是有害的,A2牛奶是有益的。尽管没有支持这些说法的临床证据,但在没有现场正确识别的情况下,牛奶以A2牛奶的名义以高成本销售。为了向消费者和生产商解决这个问题,科学家们可以排除A1和A2牛奶的可能性。在本研究中,TANUVAS A1A2检测试剂盒用于具有干血或乳斑的A1或A2型牛奶。从蒂鲁奇拉帕利区不同地方的农场和个体农民那里采集了31份样本。所有样本均使用基于引物的PCR试剂盒TANUVAS A1A2检测试剂盒进行分析。本研究表明,20%的杂交奶牛、78.95%的本地品种奶牛、66.67%的非描述性奶牛和100%的水牛具有A2A2基因型。这项研究的结果表明,在申请A2牛奶之前,牛奶需要进行适当的测试,如TANUVAS A1A2检测。
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INDIAN JOURNAL OF DAIRY SCIENCE
INDIAN JOURNAL OF DAIRY SCIENCE AGRICULTURE, DAIRY & ANIMAL SCIENCE-
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