M. Gallagher-Jones, C. Glynn, D. Boyer, M. Martynowycz, Evelyn Hernandez, Jennifer Miao, Chih-Te Zee, I. Novikova, Lukasz Goldschmidt, Heather T Mcfarlane, G. Helguera, James E. Evans, M. Sawaya, D. Cascio, D. Eisenberg, T. Gonen, José A. Rodríguez
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引用次数: 15
Abstract
The atomic structure of the infectious, protease-resistant, β-sheet-rich and fibrillar mammalian prion remains unknown. Through the cryo-EM method MicroED, we reveal the sub-angstrom-resolution structure of a protofibril formed by a wild-type segment from the β2–α2 loop of the bank vole prion protein. The structure of this protofibril reveals a stabilizing network of hydrogen bonds that link polar zippers within a sheet, producing motifs we have named ‘polar clasps’.
期刊介绍：
Nature Structural & Molecular Biology is a monthly journal that focuses on the functional and mechanistic understanding of how molecular components in a biological process work together. It serves as an integrated forum for structural and molecular studies. The journal places a strong emphasis on the functional and mechanistic understanding of how molecular components in a biological process work together. Some specific areas of interest include the structure and function of proteins, nucleic acids, and other macromolecules, DNA replication, repair and recombination, transcription, regulation of transcription and translation, protein folding, processing and degradation, signal transduction, and intracellular signaling.
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