Regulatory effect of mechanical vibration on fibroblast growth factor 23 antibody during fracture healing in ovariectomized rats

Abstract

Objective To investigate the regulatory effect of mechanical vibration intervention on endocrine hormone fibroblast growth factor23 (FGF23) in ovariectomized fracture rats. Methods A model of postmenopausal osteoporosis was established in 45 female rats, and the animals were randomly divided into three groups: Sham operation group (Sham, n=15), ovariectomized fracture model group (OVX, n=15) and ovariectomized fracture vibration group (OVX-V, n=15). The rats in OVX-V group were subjected to whole-body vibration at a frequency of 35 Hz, a vibration amplitude of 2 mm, and an acceleration of 0.5 g, 20 min/d and 5 t/w, 5 days after fracture surgery. The rats in Sham group and OVX group were placed on the vibrating table for 20 min at 5th day after the operation, and no vibration was performed. Western blotting was used to detect the expression of FGF23 protein in the fracture end of rats. SPSS 19.0 statistical software was used to analyze the results. The results were expressed as mean±standard deviation (Mean±SD). Comparisons between groups were analyzed by one-way ANOVA. Results The protein expression of FGF23 in the fracture end of OVX-V group was significantly lower than that in OVX group at 2nd, 4th and 6th week [(0.846±0.012, 1.315±0.021, 1.315±0.021) vs. (0.703±0.009, 0.466±0.011, 0.380±0.005), P<0.01]. . The protein expression of FGF23 in the fracture end of OVX group was significantly higher than that in Sham group at 2nd, 4th and 6th week [(0.168±0.004, 0.321±0.003, 0.417±0.009) vs. (0.846±0.012, 1.315±0.021, 1.315±0.021), P<0.01), showing an increasing trend, suggesting that mechanical vibration can reduce the expression level of FGF23 in the bone tissue of oarotomy rats. Conclusion Mechanical vibration can reduce the expression of FGF23 in bone tissue, promote osteoblast differentiation, regulate bone mineralization and promote postmenopausal osteoporosis fracture healing. Key words: Mechanical vibration; Osteoporosis; Fracture; Endocrine hormones; Fibroblast growth factor23