Effect of Newly Characterized Probiotic Lactobacilli on Weight Gain, Immunomodulation and Gut Microbiota of Campylobacter jejuni Challenged Broiler Chicken

IF 3.8 3区农林科学 Q1 VETERINARY SCIENCES Pakistan Veterinary Journal Pub Date : 2019-10-01 DOI:10.29261/pakvetj/2019.051

Madiha Khan

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引用次数: 11

Abstract

Received: Revised: Accepted: Published online: January 08, 2019 February 12, 2019 February 13, 2019 April 06, 2019 The aim of the present study was to determine the competitive exclusion of Campylobacter jejuni in poultry gut by three potential probiotic lactobacilli strains. A total of 135 birds of one day age were randomly divided into nine groups. The groups of prevention model received respective strains from day 1-35 and treatment model received lactobacilli from day 15-35 (after challenging with C. jejuni). These groups were given lactobacilli (~10 CFUs) while challenged with C. jejuni on day 14 by oral gavage (10 CFUs/bird). There were three control groups including A, B and I. Negative control (A) did not receive any treatment, C. jejuni was given to group B and group I was given Enrofloxacin formulation. Cloacal swabs were collected from birds of each group before and after challenge while the ceca were collected from birds after slaughtering (on day 35) for enumeration of aerobic bacteria, coliform, C. jejuni, lactobacilli and Bifidobacterium on selective agar plates. Effect of lactobacilli on weight gain and New Castle Disease vaccine (NDV) titer were also evaluated. Probiotic strain Lactobacillus gallinarum PL 53 considerably decreased log10 values of aerobic plate count (3.19±0.66), coliform count (2.83±0.22) and C. jejuni (3.98±0.77) in poultry. The probiotics also enhanced Lactobacillus and Bifidobacterium spp counts (~2-3 log increase). Treatment group C had maximum weight gain (1994±188.32g) and geometric mean titer (274.4) on day 28. The results of in-vivo experiments concluded that probiotic administration may be effective for targeted mitigation of C. jejuni in broiler birds. ©2019 PVJ. All rights reserved

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新型益生菌乳酸杆菌对空肠弯曲杆菌挑战肉鸡增重、免疫调节和肠道微生物群的影响

接收：修订：接受：在线发布：2019年1月8日2019年2月12日2019年02月13日2019年04月06日本研究的目的是通过三种潜在的益生菌乳酸杆菌菌株确定家禽肠道中空肠弯曲杆菌的竞争性排除。将135只一日龄的鸟类随机分为9组。预防模型组从第1-35天接受各自的菌株，治疗模型从第15-35天接受乳酸杆菌（用空肠弯曲菌攻击后）。这些组在第14天用空肠弯曲菌经口灌胃攻击时给予乳酸杆菌（~10 CFU）（10 CFU/只）。对照组分为A、B和I三组。阴性对照组（A）未接受任何治疗，B组给予空肠弯曲菌，I组给予恩诺沙星制剂。在攻击前后从每组鸟类中采集泄殖腔拭子，而在屠宰后（第35天）从鸟类中采集盲肠，在选择性琼脂平板上计数需氧菌、大肠杆菌、空肠弯曲菌、乳酸杆菌和双歧杆菌。还评估了乳酸杆菌对体重增加和新城疫疫苗（NDV）滴度的影响。益生菌菌株鸡乳杆菌PL 53显著降低了家禽需氧菌平板计数（3.19±0.66）、大肠菌群计数（2.83±0.22）和空肠弯曲菌（3.98±0.77）的log10值。益生菌还提高了乳酸杆菌和双歧杆菌的计数（约2-3 log增加）。治疗组C在第28天出现最大体重增加（1994±188.32g）和几何平均滴度（274.4）。体内实验结果表明，益生菌给药可能对肉鸡空肠弯曲菌的靶向缓解有效。©2019 PVJ。保留所有权利

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来源期刊

Pakistan Veterinary Journal 兽医-兽医学

CiteScore

4.20

自引率

13.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Pakistan Veterinary Journal (Pak Vet J), a quarterly publication, is being published regularly since 1981 by the Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan. It publishes original research manuscripts and review articles on health and diseases of animals including its various aspects like pathology, microbiology, pharmacology, parasitology and its treatment. The “Pak Vet J” (www.pvj.com.pk) is included in Science Citation Index Expended and has got 1.217 impact factor in JCR 2017. Among Veterinary Science Journals of the world (136), “Pak Vet J” has been i) ranked at 75th position and ii) placed Q2 in Quartile in Category. The journal is read, abstracted and indexed internationally.