Discrimination of Wild and Hatchery-Reared Black Porgy Acanthopagrus schlegelii Using Otolith Elements Analysis of Magnesium and Manganese

Abstract

Due to the decreasing resources of both inshore and offshore fisheries, many organizations have released fry to enhance their stock in recent years. The discrimination between wild and hatchery-reared individuals must be performed to assess the efficiency of the release. Fresh groundwater is generally used by hatchers in southwesternTaiwanto decrease salinity and to promote the growth of larvae prior to feeding; thus, the elemental composition of the otolith may be different in stocks, and this difference may be used for identification. This study used Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICPMS) to analyze the trace elements in the nuclei of otoliths from black porgy Acanthopagrus schlegelii, including six hatchery reared specimens from the Tainan County, three recaptured marked and ten captured unmarked specimens from the Miaoli County in Northwestern coast of Taiwan. Among the six hatchery and three recaptured marked specimens, which ranged from 4.9 to23.4 cmin body length, the nucleus zone (relative to within5 cmin size growth) of the otoliths showed higher magnesium concentrations than that observed on both edges. The distribution was similar to an inverse “V” shape. The otoliths also showed lower manganese concentrations near the nucleus and had higher values near the edges, resulting in a distribution similar to a “U” shape. According to the appeared shape of Mg2+/Ca2+ and Mn2+/Ca2+ ratio in the central area of the nucleus zone, this study determined six specimens which were from hatchery reared and three specimens were from wild among ten unmarked specimens captured from the Northwestern coast of Taiwan. Under the conditions used in this study, the absorption of calcium into the otolith was active and required energy in the seawater and freshwater, while the incorporation of magnesium and manganese was passive and was primarily affected by diffusion.