{"title":"Spectro-chemometric determination of panthenol enantiomeric excess in pharmaceutical products","authors":"R. Poláček, A. Kleinová, P. Májek","doi":"10.2478/acs-2020-0020","DOIUrl":null,"url":null,"abstract":"Abstract Antibiotic, soothing and healing properties of panthenol are exploited in various pharmaceutical and cosmetic products. Only D-panthenol is biologically active while its L-form might counteract the biological effectiveness of the D-enantiomer. Hydrating and moisturizing effects are exhibited by both enantiomers. Therefore, it is necessary to develop a rapid and cheap method for the determination of panthenol enantiomeric excess in pharmaceutical preparations. In this study, b-cyclodextrin was used as a chiral selector for the recognition of panthenol enantiomers. Inclusion complexes formed by b-cyclodextrin and the analyte showed small differences in NIR and UV/VIS spectra compared by chemometric assessment. Based on the figure of merit and model characteristics, PLS calibration model in the selected range of NIR spectra is preferred. UV/VIS spectrometry has the disadvantage of complicated sample preparation compared to NIR spectrometry. Results reached by both proposed methods were in good agreement. Statistical investigation of the results (by Student t-test and Fisher F-test) confirmed that the proposed methods are comparable and applicable to chiral analysis of panthenol pharmaceutical preparations.","PeriodicalId":7088,"journal":{"name":"Acta Chimica Slovaca","volume":"13 1","pages":"23 - 29"},"PeriodicalIF":0.9000,"publicationDate":"2020-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Chimica Slovaca","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/acs-2020-0020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 1
Abstract
Abstract Antibiotic, soothing and healing properties of panthenol are exploited in various pharmaceutical and cosmetic products. Only D-panthenol is biologically active while its L-form might counteract the biological effectiveness of the D-enantiomer. Hydrating and moisturizing effects are exhibited by both enantiomers. Therefore, it is necessary to develop a rapid and cheap method for the determination of panthenol enantiomeric excess in pharmaceutical preparations. In this study, b-cyclodextrin was used as a chiral selector for the recognition of panthenol enantiomers. Inclusion complexes formed by b-cyclodextrin and the analyte showed small differences in NIR and UV/VIS spectra compared by chemometric assessment. Based on the figure of merit and model characteristics, PLS calibration model in the selected range of NIR spectra is preferred. UV/VIS spectrometry has the disadvantage of complicated sample preparation compared to NIR spectrometry. Results reached by both proposed methods were in good agreement. Statistical investigation of the results (by Student t-test and Fisher F-test) confirmed that the proposed methods are comparable and applicable to chiral analysis of panthenol pharmaceutical preparations.