THE DEVELOPMENT OF MEMBRANOUS PATHOLOGY OF HEPATOCYTES THE INFLUENCE OF INTOXICATION

Abstract

The study was performed at the Department of Clinical Pathophysiology, Topographical Anatomy and Operative Surgery at Kharkiv Medical Academy of Postgraduate Education as a part of research project "Radiotoxins' pathochemical mechanisms and methods of early diagnostics and correction", state registration No. 0117U000589. We studied the subtoxic effect of small doses of sodium fluoride on the activity of microsomal hepatocytes on 30 Wistar rats' populations in subacute experiment. The intensity of lipid peroxidation (LPO) in liver of rats which were administered sodium fluoride orally for a long time at doses of 1/10 and 1/100 LD50, was evaluated by the content of its molecular products - diene conjugates (DC), and MDA-reagents Schiff bases. We found that oral administration of sodium fluoride to rats at doses of 1/10 and 1/100 LD50 promotes a statistically significant increase (r≤0,002) relative to the control group of animals in DC content during the entire period of observation. At a dose 1/10 LD50 we observed the most significant increase in this indicator on the 10th day of the experiment - at 265%, and at a dose 1/100 LD50 – on the 20th day an average of 234%. In rats’ liver by the action of subtoxical dose of sodium fluoride at a dose of 1/10 LD50, starting from the 20th day, we detected a gradual increase (r≤0.001) of TBA-reagents relative to control - for 27, 41, 78, 133%. Secondary end products and lipid peroxidation, which are defined under the long-term of sodium fluoride, somehow contribute to the disruption of the microstructure of hepatocytes membranes, their permeability, reduce their division, regeneration and inhibition of mitochondrial respiratory chain enzymes and microsomal monooxygenase system.