Microalgae growth and diversity in anaerobic digestate compared to synthetic media

IF 14.4 Q1 ENERGY & FUELS Biofuel Research Journal-BRJ Pub Date : 2022-03-01 DOI:10.18331/brj2022.9.1.2
Hande Ermis, Ünzile Güven-Gülhan, Tunahan Çakır, M. Altınbaş
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引用次数: 10

Abstract

Economizing microalgal cultivation is a considerable milestone targeted by efforts put into microalgal biorefineries. In light of that, the present study was aimed to explore the potential of using anaerobic liquid digestate (ALD) as culture media to grow microalgae and compared it with three different synthetic media (i.e., N8, BBM, and M8) in terms of biomass yield, fatty acid composition, and nutrient utilization/recovery. Moreover, a mixed culture of wild-type microalgae was employed in this study owing to the ability of mixed cultures to survive extreme conditions, eliminating the risk of losing the culture easily, as it mostly happens with pure cultures. The highest nutrient yield coefficients were achieved when the mixed microalgae culture was cultivated in ALD, where the yield coefficient for nitrogen (YN) and yield coefficient for phosphorus (YP) were 10.7 mg biomass mg-1 N and 98 mg biomass mg-1 P, respectively. The highest lipid content (34%) and the highest concentrations of C16:0 (114 mg L-1) and C18:0 (60.9 mg L-1) were also recorded when the mixed microalgae culture was cultivated in ALD. Furthermore, the polyunsaturated fatty acids (PUFA) content also increased significantly in ALD, a beneficial phenomenon as PUFAs in microalgae allow them to adapt more effectively to extreme conditions. Based on the microbial community analysis performed using the multi-marker metabarcoding approach, Diphylleia rotans, Synechocystis PCC-6803, Cyanobium gracile PCC 6307, and Chlorella sorokiniana were identified as the most abundant species in the ALD growth. Overall, based on the findings of the present study, ALD could be used as a promising cultivation medium for microalgae, offering a process integration approach to combine anaerobic digestion and algae cultivation as an effective way to simultaneously treat the high-strength dark-colored ALD and valorize it into profitable byproducts.
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与合成培养基相比,厌氧消化液中微藻的生长和多样性
节约微藻培养是微藻生物炼制工作的一个重要里程碑。有鉴于此,本研究旨在探索使用厌氧液体消化物(ALD)作为培养基培养微藻的潜力,并将其与三种不同的合成培养基(即N8、BBM和M8)在生物量产量、脂肪酸组成和营养利用/回收方面进行比较。此外,本研究采用了野生型微藻的混合培养物,因为混合培养物能够在极端条件下生存,消除了容易失去培养物的风险,就像大多数纯培养物一样。当混合微藻培养物在ALD中培养时,获得了最高的养分产量系数,其中氮产量系数(YN)和磷产量系数(YP)分别为10.7mg生物质mg-1N和98mg生物质mg-1 P。当在ALD中培养混合微藻培养物时,还记录了最高的脂质含量(34%)和最高浓度的C16:0(114mg L-1)和C18:0(60.9mg L-1)。此外,ALD中的多不饱和脂肪酸(PUFA)含量也显著增加,这是一种有益的现象,因为微藻中的PUFA使它们能够更有效地适应极端条件。基于使用多标记代谢编码方法进行的微生物群落分析,轮状白喉杆菌、聚胞囊菌PCC-6803、薄氰藻PCC 6307和索氏小球藻被确定为ALD生长中最丰富的物种。总之,根据本研究的发现,ALD可以作为一种很有前途的微藻培养基,提供了一种将厌氧消化和藻类培养相结合的过程集成方法,作为同时处理高强度深色ALD并将其增值为有利可图的副产品的有效方法。
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来源期刊
CiteScore
22.10
自引率
1.50%
发文量
15
审稿时长
8 weeks
期刊介绍: Biofuel Research Journal (BRJ) is a leading, peer-reviewed academic journal that focuses on high-quality research in the field of biofuels, bioproducts, and biomass-derived materials and technologies. The journal's primary goal is to contribute to the advancement of knowledge and understanding in the areas of sustainable energy solutions, environmental protection, and the circular economy. BRJ accepts various types of articles, including original research papers, review papers, case studies, short communications, and hypotheses. The specific areas covered by the journal include Biofuels and Bioproducts, Biomass Valorization, Biomass-Derived Materials for Energy and Storage Systems, Techno-Economic and Environmental Assessments, Climate Change and Sustainability, and Biofuels and Bioproducts in Circular Economy, among others. BRJ actively encourages interdisciplinary collaborations among researchers, engineers, scientists, policymakers, and industry experts to facilitate the adoption of sustainable energy solutions and promote a greener future. The journal maintains rigorous standards of peer review and editorial integrity to ensure that only impactful and high-quality research is published. Currently, BRJ is indexed by several prominent databases such as Web of Science, CAS Databases, Directory of Open Access Journals, Scimago Journal Rank, Scopus, Google Scholar, Elektronische Zeitschriftenbibliothek EZB, et al.
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