PHYLOGENETIC ANALYSIS OF SOME SPECIES OF CYPERACEAE BASED ON DNA INTERNAL TRANSCRIBED SPACERS (ITS) IN IRAQ

IF 1.6 Q3 PLANT SCIENCES Sabrao Journal of Breeding and Genetics Pub Date : 2023-06-30 DOI:10.54910/sabrao2023.55.3.9

B. Hamid, Zta AL-RUFAYE, Hala S. Khaleel, AR Abidukadhim

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Abstract

Cyperaceae is the third-largest monocot family and has ecological and commercial significance worldwide. It is also a perfect model family for evolutionary research due to species diversity and the wide range of variations in lineage diversity. Using a nuclear region internal transcribed spacer (ITS), the current study attempted to identify the three species (Carex otrubae, Bolboschoenus fluviatilis, and Eleocharis palustris) and examine their relationships within the Cyperaceae family. In 2021, gathering of samples transpired from various locations along the Al-Hussainiya River in Iraq and the National Herbarium. According to the findings, the length of the fragments sequenced ranged between 750 and 1000 bp. Results also showed the sequence alignments between the two species (Carex otrubae and Bolboschoenus fluviatilis) have similarities and differences with those deposited in the Genome Bank. However, the Eleocharis palustris showed complete similarity in all regions of the nitrogenous base sequences with species deposited in the Genome Bank.

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基于DNA内转录间隔区（ITS）的伊拉克莎草科几种植物系统发育分析

莎草科是第三大单子叶植物科，在世界范围内具有重要的生态和商业意义。由于物种多样性和谱系多样性的广泛变化，它也是进化研究的完美模式家族。利用核区内部转录间隔区（ITS），目前的研究试图鉴定这三个物种（苔草、Bolboschoenus fluviatilis和Eleocharis palustris），并检查它们在莎草科中的关系。2021年，从伊拉克胡塞尼亚河和国家植物标本馆的不同地点采集了样本。根据研究结果，测序片段的长度在750到1000个碱基之间。结果还表明，这两个物种（苔草属和河苔草属）之间的序列比对与保存在基因组库中的序列比对有相似之处，也有不同之处。然而，沼泽Eleocharis在含氮碱基序列的所有区域都与基因组库中的物种表现出完全相似。

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来源期刊

Sabrao Journal of Breeding and Genetics 农林科学-奶制品与动物科学

CiteScore

1.90

自引率

50.00%

发文量

期刊介绍： The SABRAO Journal of Breeding and Genetics is an international journal of plant breeding and genetics research and was first published in 1969. It is the official publication of the Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO). Its objectives are to: promote the international exchange of research information on plant breeding and genetics, by describing new research findings, or ideas of a basic or practical nature; and be a medium for the exchange of ideas and news regarding members of the Society. The Journal gives priority to articles that are of direct relevance to plant breeders and with emphasis on the Asian region. Invited for publication are research articles, short communications, methods, reviews, commentaries, and opinion articles. Scientific contributions are refereed and edited to international standards. The journal publishes articles for SABRAO members mainly. The Journal preferred strongly that at least one author should be a current member of the Society. Non-members may also publish in the journal.