Associations of INPPL1 (+1893CC/AA and + 2945AA/GG) exonic polymorphisms with the risk of type 2 diabetes mellitus in North Indian population: A case control study

IF 0.8 Q4 GENETICS & HEREDITY Meta Gene Pub Date : 2021-09-01 DOI:10.1016/j.mgene.2021.100929
Jaswinder Singh , Vikas Kumar , Kiran Bala , Ashish Aneja , Jasbir Singh
{"title":"Associations of INPPL1 (+1893CC/AA and + 2945AA/GG) exonic polymorphisms with the risk of type 2 diabetes mellitus in North Indian population: A case control study","authors":"Jaswinder Singh ,&nbsp;Vikas Kumar ,&nbsp;Kiran Bala ,&nbsp;Ashish Aneja ,&nbsp;Jasbir Singh","doi":"10.1016/j.mgene.2021.100929","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and aim</h3><p><em>INPPL1</em> gene encodes a lipid phosphatase Src homology 2-containing 5′-inositol phosphatase 2 (SHIP2) which is involved in the negative regulation of insulin signaling. In the light of several association studies of <em>INPPL1</em> gene polymorphisms with T2DM, and the absence of association studies from India, present study was conducted to assess the association of two SNPs of <em>INPPL1</em> gene with T2DM risk in North Indian population.</p></div><div><h3>Material and method</h3><p>Two single nucleotide polymorphism (SNPs) (+1893CC/AA and + 2945AA/GG) in <em>INPPL1</em> gene were genotyped in total of 270 subjects with PCR-RFLP genotyping method.</p></div><div><h3>Results</h3><p>It was observed that, out of the two SNPs, only +1893CC/AA was found to be significantly associated with T2DM and frequency of A allele (C vs A, <em>p</em> = 0.002) has been found to be significantly higher in T2DM cases. Strong linkage disequilibrium was observed between two SNPs as assessed through D′ and r<sup>2</sup> (D′ = 0.984, r<sup>2</sup> = 0.009) and AG haplotype (OR = 3.59, 95% CI (1.59–9.80), <em>p</em> = 0.0015) was associated with increased risk of T2DM while CG haplotype (OR = 0.54, 95% CI (0.33–0.88) <em>p</em> = 0.012) significantly decreases the risk of developing T2DM. Regression analysis showed that SNP +1893CC/AA is associated with T2DM risk when adjusted for clinical/demographic variables.</p></div><div><h3>Conclusion</h3><p>Results showed that <em>INPPL1</em> gene polymorphism +1893CC/AA may increase susceptibility to T2DM and ‘A' allele might be serving as a risk factor in development of T2DM in Indian population.</p></div>","PeriodicalId":38190,"journal":{"name":"Meta Gene","volume":"29 ","pages":"Article 100929"},"PeriodicalIF":0.8000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.mgene.2021.100929","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Meta Gene","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214540021000803","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 1

Abstract

Background and aim

INPPL1 gene encodes a lipid phosphatase Src homology 2-containing 5′-inositol phosphatase 2 (SHIP2) which is involved in the negative regulation of insulin signaling. In the light of several association studies of INPPL1 gene polymorphisms with T2DM, and the absence of association studies from India, present study was conducted to assess the association of two SNPs of INPPL1 gene with T2DM risk in North Indian population.

Material and method

Two single nucleotide polymorphism (SNPs) (+1893CC/AA and + 2945AA/GG) in INPPL1 gene were genotyped in total of 270 subjects with PCR-RFLP genotyping method.

Results

It was observed that, out of the two SNPs, only +1893CC/AA was found to be significantly associated with T2DM and frequency of A allele (C vs A, p = 0.002) has been found to be significantly higher in T2DM cases. Strong linkage disequilibrium was observed between two SNPs as assessed through D′ and r2 (D′ = 0.984, r2 = 0.009) and AG haplotype (OR = 3.59, 95% CI (1.59–9.80), p = 0.0015) was associated with increased risk of T2DM while CG haplotype (OR = 0.54, 95% CI (0.33–0.88) p = 0.012) significantly decreases the risk of developing T2DM. Regression analysis showed that SNP +1893CC/AA is associated with T2DM risk when adjusted for clinical/demographic variables.

Conclusion

Results showed that INPPL1 gene polymorphism +1893CC/AA may increase susceptibility to T2DM and ‘A' allele might be serving as a risk factor in development of T2DM in Indian population.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
INPPL1(+1893CC/AA和+2945AA/GG)外显子多态性与北印度人群2型糖尿病风险的相关性:一项病例对照研究
背景和目的:ppl1基因编码脂质磷酸酶Src同源2-含5 ' -肌醇磷酸酶2 (SHIP2),参与胰岛素信号的负向调控。鉴于已有几项关于INPPL1基因多态性与T2DM的关联研究,而印度缺乏相关研究,本研究旨在评估印度北部人群中INPPL1基因的两个snp与T2DM风险的关系。材料与方法采用PCR-RFLP基因分型方法对270例受试者进行INPPL1基因2个单核苷酸多态性(+1893CC/AA和+ 2945AA/GG)分型。结果2个snp中,只有+1893CC/AA与T2DM显著相关,而A等位基因的频率(C vs A, p = 0.002)在T2DM患者中显著较高。通过D′和r2 (D′= 0.984,r2 = 0.009)和AG单倍型(OR = 3.59, 95% CI (1.59-9.80), p = 0.0015)与T2DM发病风险增加相关,而CG单倍型(OR = 0.54, 95% CI (0.33-0.88) p = 0.012)显著降低T2DM发病风险。回归分析显示,在调整临床/人口统计学变量后,SNP +1893CC/AA与T2DM风险相关。结论INPPL1基因多态性+1893CC/AA可能增加印度人群对T2DM的易感性,“A”等位基因可能是印度人群发生T2DM的危险因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Meta Gene
Meta Gene Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
1.10
自引率
0.00%
发文量
20
期刊介绍: Meta Gene publishes meta-analysis, polymorphism and population study papers that are relevant to both human and non-human species. Examples include but are not limited to: (Relevant to human specimens): 1Meta-Analysis Papers - statistical reviews of the published literature of human genetic variation (typically linked to medical conditionals and/or congenital diseases) 2Genome Wide Association Studies (GWAS) - examination of large patient cohorts to identify common genetic factors that influence health and disease 3Human Genetics Papers - original studies describing new data on genetic variation in smaller patient populations 4Genetic Case Reports - short communications describing novel and in formative genetic mutations or chromosomal aberrations (e.g., probands) in very small demographic groups (e.g., family or unique ethnic group). (Relevant to non-human specimens): 1Small Genome Papers - Analysis of genetic variation in organelle genomes (e.g., mitochondrial DNA) 2Microbiota Papers - Analysis of microbiological variation through analysis of DNA sequencing in different biological environments 3Ecological Diversity Papers - Geographical distribution of genetic diversity of zoological or botanical species.
期刊最新文献
Severity of coronavirus disease 19: Profile of inflammatory markers and ACE (rs4646994) and ACE2 (rs2285666) gene polymorphisms in Iraqi patients MicroRNAs based regulation of cytokine regulating immune expressed genes and their transcription factors in COVID-19 Interleukin-37 gene polymorphism and susceptibility to coronavirus disease 19 among Iraqi patients Is there any relationship between serum zinc levels and angiotensin-converting enzyme 2 gene expression in patients with coronavirus disease 2019? Genetic analysis of IL4 (rs2070874), IL17A (rs2275913), and IL33 (rs7044343) polymorphisms in Iraqi multiple sclerosis patients by using T-plex real-time PCR method
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1