Genetic diversity of Salvia tomentosa Miller (Lamiaceae) species using Touch-down Directed Amplification of Minisatellite DNA (Td-DAMD) molecular markers

Q3 Agricultural and Biological Sciences Acta Biologica Szegediensis Pub Date : 2020-05-27 DOI:10.14232/ABS.2019.2.135-141
B. Saleh
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引用次数: 2

Abstract

Salvia tomentosa Miller (Lamiaceae) a Mediterranean species has an important role in various pharmacological applications. To reveal genetic relationships among S. tomentosa natural populations, 35 samples were collected from different regions of Syria. Touch-down Directed Amplification of Minisatellite DNA (Td-DAMD) markers have been investigated for this goal. Td-DAMD assay produced 158 total bands of which 131 (82.911%) were polymorphic with a mean polymorphic information content (PIC) value of 0.264 and a mean marker index (MI) value of 2.269. Clustering profile based on TdDAMD data showed that samples were grouped into two main clusters; the first cluster included Lattakia samples which split into two subclusters regardless their altitudes over the sea level. Whereas, the second cluster included Tartous and Hama samples. Td-DAMD assay successfully discriminate among the tested 35 samples belonged to the S. tomentosa natural population.
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基于微卫星DNA (Td-DAMD)分子标记的毛毛鼠尾草遗传多样性研究
毛毛鼠尾草(Salvia tomentosa Miller, Lamiaceae)是一种地中海植物,具有重要的药理作用。为了揭示毛毛沙棘自然种群间的遗传关系,从叙利亚不同地区采集了35份样本。为此,我们研究了微卫星DNA (Td-DAMD)标记的定向触地扩增技术。Td-DAMD检测共获得158个条带,其中131个(82.911%)为多态性条带,平均多态性信息含量(PIC)值为0.264,平均标记指数(MI)值为2.269。基于TdDAMD数据的聚类曲线显示,样本被分为两个主要聚类;第一组包括拉塔基亚的样本,无论海拔多高,它们都分成了两个亚组。然而,第二个聚类包括Tartous和Hama样本。Td-DAMD法成功地区分了35份毛毛鼠自然种群的样品。
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来源期刊
Acta Biologica Szegediensis
Acta Biologica Szegediensis Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.00
自引率
0.00%
发文量
14
期刊介绍: Acta Biologica Szegediensis (ISSN 1588-385X print form; ISSN 1588-4082 online form), a member of the Acta Universitatis Szegediensis family of scientific journals (ISSN 0563-0592), is published yearly by the University of Szeged. Acta Biologica Szegediensis covers the growth areas of modern biology and publishes original research articles and reviews, involving, but not restricted to, the fields of anatomy, embryology and histology, anthropology, biochemistry, biophysics, biotechnology, botany and plant physiology, all areas of clinical sciences, conservation biology, ecology, genetics, microbiology, molecular biology, neurosciences, paleontology, pharmacology, physiology and pathophysiology, and zoology.
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